Healthy
Conditions
Keywords
Protein, Plant-based protein, Metabolism, Somatotrophic Axis
Brief summary
The goal of this interventional study is to learn about the effects of different proteins (from animals or plants) on the metabolism in healthy, normal weight women between 18-45 year who are not on hormonal birth-control. The main questions the study aims to answer are: 1. How do different proteins affect biological markers of the metabolism and which protein has the strongest effect? 2. How are the different proteins digested and taken up from our body? Participants will drink four different shakes on four different study days in a random order. Three of them contain each a different protein and one does not contain protein. Right before drinking the shake and on 11 timepoints after drinking the shake blood samples will be drawn.
Interventions
DIETARY_SUPPLEMENTIsolated Pea Protein
Participants will consume the protein supplement dissolved in water in an opaque mug wearing a nose clip.
DIETARY_SUPPLEMENTIsolated Whey Protein
Participants will consume the protein supplement dissolved in water in an opaque mug wearing a nose clip.
DIETARY_SUPPLEMENTIsolated Faba Bean Protein
Participants will consume the protein supplement dissolved in water in an opaque mug wearing a nose clip.
DIETARY_SUPPLEMENTPlacebo
Participants will consume the placebo in an opaque mug wearing a nose clip.
Sponsors
Örebro University, Sweden
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
TRIPLE (Subject, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
* Females * Age: 18-45 years * Body mass index: 18.5 \< 25 kg/m2 * Signed informed consent
Exclusion criteria
* Use of hormonal contraceptives * Pregnancy or breastfeeding * Diabetes mellitus or any other metabolic disease that might affect the results of this study * Gastric surgery that affects digestion and uptake of protein * Any clinically significant present or past disease/condition and medication which in the investigator's opinion could interfere with the results of the trial
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Release of Growth Hormone | 0-3 hours after consumption of test drink (on all 4 study days) | The primary outcome is defined as the postprandial release of growth hormone during three hours after oral ingestion of plant-protein test drinks vs. an animal-protein test drink measured as incremental area under the curve. Growth hormone will be measured in blood samples. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Release of Hormones Related to the Somatotrophic Axis | 0-3 hours after consumption of test drink (on all 4 study days) | We will measure different hormones related to the somatotrophin axis in collected blood samples and investigate if there are differences between different protein-sources. |
| Kinetics of Amino Acid Uptake | 0-3 hours after consumption of test drink (on all 4 study days) | We will measure amino acids in collected blood samples to investigate dynamics and kinetics of protein digestion/uptake of different protein sources. |
Countries
Sweden
Outcome results
None listed