Endodontic Disease, Periapical Diseases, Periapical; Infection, Root Canal Infection
Conditions
Keywords
apical shaping, Droplet Digital PCR, irrigation activation, microbial load, root canal cleanliness
Brief summary
The goal of this observational study is to define the role of apical shaping and irrigation activation on root canal cleanliness. The main questions it aims to answer are: * does the effectiveness of irrigation activation depends on apical shaping? * can a similar success be achieved by increasing apical shaping without irrigation activation Participants will \[describe the main tasks participants will be asked to do, treatments they'll be given and use bullets if it is more than 2 items\].
Detailed description
The aim of this study is to evaluate the effect of apical enlargement with two different diameters on the intraradicular microbiota of teeth with chronic apical periodontitis lesion before and after passive ultrasonic irrigation activation using ddPCR analysis in vivo.The study was planned as a randomized controlled clinical trial. We are planning to include 24 patients who was referred to the Istanbul Medipol University Faculty of Dentistry Endodontic Clinic with the need for root canal treatment, with asymptomatic chronic apical periodontitis. 24 patients will randomly be assigned to two groups according to the apical shaping size during root canal treatment: 25/.04 and 35/.04. Microbiological sampling of the teeth will be done with paper cones in three steps during the root canal treatment: 1. Sterility control samples (SR1) from the tooth surface after cavity preparation, 2. The first microbiological sample (S1) from the root canal after the initial file reaches the working length. 3. The second microbiological sample (S2) from the root canal after the shaping and irrigation of the root canal is finished. 4. The third microbiological sample (S3) from the root canal after the irrigation activation in the root canal is finished.The samples will be sent to the genetic analysis laboratory for further analysis in the cold chain using a Droplet Digital PCR.
Interventions
PROCEDUREapical shaping size
24 patients will randomly be assigned to two groups according to the apical shaping size during root canal treatment: 25/.04 and 35/.04. Microbiological sampling of the teeth will be done with paper cones in three steps during the root canal treatment: 1. Sterility control samples (SR1) from the tooth surface after cavity preparation, 2. The first microbiological sample (S1) from the root canal after the initial file reaches the working length. 3. The second microbiological sample (S2) from the root canal after the shaping and irrigation of the root canal is finished. 4. The third microbiological sample (S3) from the root canal after the irrigation activation in the root canal is finished.The samples will be sent to the genetic analysis laboratory for further analysis in the cold chain using a Droplet Digital PCR.
Sponsors
Istanbul Medipol University Hospital
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 65 Years
Healthy volunteers
Yes
Inclusion criteria
* Patients between the ages of 18-65 * Patients complying with the ASA1 and ASA2 classification (American Society of Anesthesiology) * Patients who has molar teeth with radiolucent lesions in the periapical region * The volunteer himself/herself or his/her legal representative wants to participate in the research by signing the 'Voluntary Consent Form'. * Being able to tolerate the treatment physically and mentally
Exclusion criteria
* Patients who have taken antibiotics/corticosteroids in the last 3 months * Patients with systemic disease * Teeth that cannot be properly isolated with a rubber dam * Teeth without coronal sealing, * Teeth with periodontal pocket depth\>4 mm and teeth with crown / root fracture. * Individuals who continue or are considering starting orthodontic treatment during the research * Pregnant and lactating individuals * The volunteer himself/herself or his/her legal representative does not want to participate in the research by signing the 'Voluntary Consent Form'. * Teeth that are damaged enough to require post-core treatment.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| apical shaping size | through study completion, an average of 1 year | does apical shaping size affect the microbial load in the root canal after shaping and irrigation finishes? microbial load after two different apical shaping size will be compared between two groups. Droplet Digital PCR will be used to measure and compare the microbial load within the root canal before and after irrigation protocol in each group to measure this outcome. |
| irrigation activation | through study completion, an average of 1 year | does irrigation activation affect the microbial load in the root canal? microbial load after chemomechanical cleaning of the root canal and after the irrigation activation will be compared. Droplet Digital PCR will be used to measure microbial load before after irrigation activation in each canal to see its effect on reduction fo microbial load within the root canal. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| the effect of apical shaping in irrigation activation | through study completion, an average of 1 year | does apical size affect the irrigation activation? the microbial load after chemomechanical cleaning of the root canal and after irrigation activation will be compared between two groups (with different apical shaping size) to analyse the effect of apical shaping on irrigation activation. Droplet Digital PCR results before and after irrigation activation in root canals with different apical size will be compared to measure this outcome. |
Countries
Turkey (Türkiye)
Contacts
Primary ContactTan Firat Eyüboglu
Outcome results
None listed