Chronic HBV Infection, Drug Withdrawal
Conditions
Keywords
transcription factor bet, chronic hepatitis B, nucleotide analogs, function cure, B cell
Brief summary
The immune mechanism of the nucleos(t)ide analogs (NAs) in inhibiting HBV replication effectively while having a low sustained virus control rate after drug withdrawal is unclear. B cell immunity and antibody response are the keys to prevent HBV reinfection and keep the virus under control. T-bet+ B, which can be regulated by IL-21, is a newly discovered major effector B cell in protection of pathogens and it is a main subtype of HBsAg-specific B cells. Thus, we suspect that T-bet+ B may play a role in ongoing controlling of the virus after withdraw of NAs in CHB patient. Based on our previous studies on CHB immunity, we use the RNAseq analyse, flow cytometry, and Elispot assay to analyze the frequency, function, and phenotype of B cells in CHB patients with different profiles after withdraw of NAs.
Interventions
DIAGNOSTIC_TESTT-bet B cell
The frequency, function, and phenotype of T-bet B cells was tested
Sponsors
Changhai Hospital
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 70 Years
Healthy volunteers
Yes
Inclusion criteria
18 to 70 years old, no gender restriction, serum HBsAg positive than 6 months, can understand and sign informed consent, good compliance.
Exclusion criteria
coinfected with other hepatotropic virus such as hepatitis C virus,hepatitis D -virus,hepatitis E and hepatitis A etc; coinfected with HIV, markers such as ceruloplasmin, anti-nuclear antibodies and anti-mitochondrial antibodies for co-existent autoimmune and metabolic liver diseases were positive, with hepatocellular carcinoma(HCC) with uncontrollable extrehepatic disease, received glucocorticoid or other immune inhibitor therapy, pregnancy
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| phenotype of B cells | PBMC were collected 0、4、12、24 week after withdraw the NAs | CD19+cells were sorted from different samples, the frequency, function, and phenotype of B cells were analyzed. |
| B cell ELISPOT assay | Cells were stimulated with R-848 (1 μg/ml) and IL-2 (10 ng/ml) for 5 days at 37 °C to aid memory B cell differentiation | Sorted B cell subsets were stimulated for 5 days following which B cell ELISPOT was conducted. |
Countries
China
Outcome results
None listed