Infertility, IVF
Conditions
Brief summary
Various to-be-discard material from IVF treatment cycle will be collect and examined, aiming to identify potential reasons of successful outcome or cycle failure.
Detailed description
Usually around 2/3 of IVF material will be discarded at the end of each treatment cycle, including immature oocytes, abnormal fertilized zygotes, poor grading embryos, spent culture medium, etc. Next generation sequencing will be performed for DNA / RNA analysis including the length and the quantity, RNA-seq will be applied for transcriptome profiling, and protein profile will be investigated in a pool sample. By applying various diagnostic tools against those to-be-discard biomaterials, we aim to identify potential underlying reasons of indicating either for an IVF failure or a successful cycle, and try to develop a non-invasive testing method for embryo quality check; better detection of the embryo implantation potential and eventually further increase the IVF clinical outcome.
Interventions
None listed
Sponsors
Chinese University of Hong Kong
Study design
Observational model
OTHER
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Inclusion criteria
* Patients attending the Assisted Reproductive Technology (ART) Unit of the Chinese University of Hong Kong for ART treatment. * Participant able to give voluntary, written, informed consent to participate in the study.
Exclusion criteria
* Patient using donor gamete in IVF treatment * Patient refuses to join and failed to giving consent for any reasons
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| DNA/RNA sequencing data | Through study completion, around 3 year | DNA / RNA analysis including the length and the quantity, RNA-seq will be applied for transcriptome profiling. Next generation sequencing will be performed to determine the sequence of DNA/RNA for our collected samples. |
| Proteomic profile | Through study completion, around 3 year | Protein profile will be investigated in a pool sample, treated samples will go for mass spectrometry (MS) data acquisition and finally destined for data analysis and interpretation. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Blastocyst formation rate | 5 days after fertilization | Number of blastocysts out of fertilized oocytes observed 5 days after fertilization |
| Clinical pregnancy rate | Around 5 weeks after embryo transfer | Clinical pregnancy confirmed by ultrasound |
| Live birth rate | Around 9 months after embryo transfer | Birth of a living infant after embryo transfer |
Countries
Hong Kong
Contacts
Primary ContactYiu Leung D Chan, DPhil
Outcome results
None listed