Assisted Reproductive Technique, Elderly, Infertility, Female, Mitochondrial
Conditions
Brief summary
The goal to this clinical trial is to study the effectiveness and safety of automitochondrial transplantation of urine derived stem cells(USCs) in in elderly patients with low prognosis after repeated IVF failure. The main questions it aims to answer are: (1）test the effectiveness and safety of automitochondrial transplantation of urine derived stem cells (2) establish intracytoplasmic sperm injection(ICSI) mitochondrial transplantation platform Participants will be asked to:1)urine collections to further culture of autologous USCs 2)ovarian stimulation、trigger eggs、ICSI with autologous mitochondria 3）embryo transfer 4)accept pregnancy follow-up at given point
Interventions
PROCEDUREAutologous USC mitochondrial oocyte transplantation
Mitochondria are isolated from urine derived stem cells from fresh middle urine of the subjects, and injected into the cytoplasm of mature oocytes together with sperm
Sponsors
Peking University People's Hospital
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
TREATMENT
Masking
NONE
Eligibility
Sex/Gender
FEMALE
Age
35 Years to No maximum
Healthy volunteers
No
Inclusion criteria
1. 1\) At least 35 years old 2. 2\) Repeated IVF failure: more than or equal to 2 egg retrieval with poor embryo quality, no transplanted embryos or failed embryo transfer (more than or equal to 4 embryos) 3. 3\) Egg number ≤9 4. 4\) Participate voluntarily and sign informed consent.
Exclusion criteria
1: Infertility caused by severe oligospermia, severe intrauterine adhesions, genital tract malformations, thyroid dysfunction and other endocrine, immune system, tumor and genetic diseases
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| live birth rate | 1year after embryo transfer | Live birth rate per embryo transfer cycle (defined as live delivery after ≧28 weeks) |
Countries
China
Outcome results
None listed