Adrenal Insufficiency, Cushing Syndrome, Hyperaldosteronism
Conditions
Brief summary
Cortisol is an essential for life hormone secreted in a pulsatile pattern on a diurnal rhythm. Given the complexity of cortisol secretion on a circadian rhythm with pulsations, current methods of cortisol measurement have limitations. Therefore, a non-invasive and ambulatory method would be useful to measure cortisol levels in real-time. The main aim of the study is to compare cortisol levels across biological fluids (sweat, saliva, interstitial fluid, and blood) in order to validate in the long term a continuous and non-invasive cortisol measurement device (currently under development).
Interventions
OTHER24 hours sampling of 4 biological fluids (blood, ISF, sweat and saliva)
24 hours sampling of 4 biological fluids: * blood: sampling every 20 minutes (through a venous catheter) * ISF: automated sampling every 20 minutes (microdialysis technique connected to U-Rhythm device) * Sweat : collection of 1 to 3 samples at 4 differents timepoints * Saliva : sampling every hour (except during the night)
OTHER24 hours sampling of ISF or sweat (after validating the correlation with blood)
24 hours sampling in an ambulatory setting
Sponsors
Centre Hospitalier Universitaire Vaudois
Study design
Observational model
OTHER
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 30 Years
Healthy volunteers
Yes
Inclusion criteria
Healthy controls: Inclusion Criteria: * age between 18 and 30 years old * normal BMI 18 to 25 kg/m2 * weight stability
Exclusion criteria
* pregnancy, breastfeeding, irregular menses * any medical treatment * any medical issues * pilocarpine allergy, or latex allergy * glaucoma or acute irritis
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Cortisol levels in 4 biologicals fluids | 24 hours | Comparison of cortisol levels in these 4 biological fluids |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Aldosterone levels in 4 biological fluids | 24 hours | Comparison of aldosterone levels in these 4 biological fluids |
| Glucocorticoid - comparison of repeated measures of glucocorticoid concentrations (cortisol, 18OHF, cortisone) profiles - over a period of 24 hours | 24 hours | Study of the diurnal rhythm and pulsatility in healthy participants (and later patients) * Diurnal rhythm: cosinor analysis. Comparison of nadir/acme of cortisol concentrations (and 18OHF, cortisone) among participants. Amplitude of the curve * Pulsatility analysis: frequency of the pulses and amplitude |
| Mineralocorticoid - comparison of repeated measures of aldosterone concentrations profiles - over a period of 24 hours | 24 hours | Study of the diurnal rhythm and pulsatility in healthy participants (and later patients) * Diurnal rhythm: cosinor analysis. Comparison of nadir/acme of aldosterone concentrations among participants. Amplitude of the curve * Pulsatility analysis: frequency of the pulses and amplitude |
Countries
Switzerland
Contacts
Primary ContactAdelina Ameti
Backup ContactNathalie Vionnet, MD-PhD
Outcome results
None listed