Infertility, Male, Klinefelter Syndrome
Conditions
Keywords
Klinefelter Syndrome, fertility preservation, male
Brief summary
Organoid Model to unravel Klinefelter Syndrome infertility Klinefelter Syndrome (KS) is characterized by the presence of an extra chromosome X in male (47,XXY), it is the most frequent genetic cause of azoospermia in adult men. The investigators will isolate and expand spermatogonial cells from KS patients, then using an organoid model investigators will compare the behavior of these Spermatogonia from KS patients when interacting with four combinations of somatic cell types incorporated in the Extra Cellular Matrix hydrogel.
Detailed description
Men with Klinefelter Syndrome candidate for TESE or microTESE (testicular sperm extraction) for azoospermia. One extra testicular biopsy (1-2 mm) will be cryopreserved and used later for the cells isolation and culture.
Interventions
PROCEDURETesticular biopsy
Testicular biopsy is a clinical procedure for extraction of testicular tissue
Sponsors
Cliniques universitaires Saint-Luc- Université Catholique de Louvain
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
MALE
Age
5 Years to 50 Years
Healthy volunteers
No
Inclusion criteria
* Klinefelter
Exclusion criteria
* Mosaicism
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Klinefelter's infertility physiopathology | Up to 60 days with intermediate time analyses at the 5th and 10 th passage of cultures. | Testicular biopsies will be digested using two steps enzymatic protocol. Cell suspensions will be in vitro cultured during 2 months. The following analyses will be performed: cells counts, cells characterisation by immunofluorescence and RTqPCR specific for the cell type, cells karyotypes. |
Countries
Belgium
Contacts
Primary ContactChristine Wyns, MD, PhD
Backup ContactMaria Grazia Giudice, MD
Outcome results
None listed