Muscle Injury, Pulsed Electromagnetic Therapy, Muscle Stretching Exercise
Conditions
Keywords
Muscle Injury, TGF-Beta 1, Fibrosis
Brief summary
In this study, a total of 69 Wistar Albino rats were used, five of which were in the preliminary study. The preliminary study was planned to determine the ideal PEMF treatment time. In the study, animals were divided into 5 groups (Control, INJ, INJ+Exercise, INJ+PEMF, INJ+Exercise+PEMF). Experimental animals were sacrificed on the 7th and 14th days to see the effects of the treatments. At the end of the experiment, genetic, histopathological, and immunohistochemical evaluations were made in the muscle tissue.
Detailed description
Purpose: The aim of this study was to evaluate the efficacy of stretching exercise and the pulsed electromagnetic field (PEMF) method in the treatment of muscle injury (INJ). Methods: In this study, a total of 69 Wistar Albino rats were used, five of which were in the preliminary study. The preliminary study was planned to determine the ideal PEMF treatment time. In the study, animals were divided into 5 groups (Control, INJ, INJ+Exercise, INJ+PEMF, INJ+Exercise+PEMF). Experimental animals were sacrificed on the 7th and 14th days to see the effects of the treatments. At the end of the experiment, genetic, histopathological, and immunohistochemical evaluations were made in the muscle tissue.
Interventions
OTHERExercise
5 day/week, two week, one session stretching exercises consisting of ten repetitions of one minute
5 day/week, two week,two sessions consisting of 180 minutes
Sponsors
Suleyman Demirel University
Study design
Allocation
RANDOMIZED
Intervention model
SINGLE_GROUP
Primary purpose
TREATMENT
Masking
NONE
Eligibility
Sex/Gender
FEMALE
Age
10 Months to 11 Months
Healthy volunteers
No
Inclusion criteria
* Healthy young wistar albino rats
Exclusion criteria
* not having any injury
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Mononuclear cell infiltration, Atrophy and necrosis, Pyknotic nucleus assesment at the end of two weeks | two week | hematoxylin-eosin staining |
| VEGF and FGF assesment at the end of two weeks | two week | immunohischemical staining |
| TGF-β1, eNOS assesment at the end of two weeks | two week | Quantitative Reverse-Transcriptase Polymerase Chain Reaction (RT-qPCR) |
Countries
Turkey (Türkiye)
Outcome results
None listed