Caffeine, Fat Burn, Genetic Predisposition
Conditions
Brief summary
Genetic polymorphism on the effect of oral caffeine intake on fat oxidation during exercise has been studied in active and healthy population performing an incremental test on a cycle ergometer with 3-min stages at workloads from 30 to 70% of maximal oxygen uptake (VO2max). Participants performed this test after the ingestion of a) placebo; b) 3 mg/kg of caffeine; c) 6 mg/kg of caffeine. Fat oxidation rate during exercise was measured by indirect calorimetry. The influence of the CYP1A2 c.-163A\>C, GSTP c.313A\>G and PGC1a polymorphisms was evaluated to determine the effects on fat oxidation during exercise
Detailed description
Caffeine is a natural stimulant with well-recognized sports performance benefits. Aside its performance-enhancing effect, caffeine has the potential of increasing fat utilization during aerobic exercise at submaximal intensities, lowering-down the contribution of carbohydrate as a fuel. This property of caffeine may provoke a glycogen-sparing effect in the skeletal muscle and liver for exercise situations where carbohydrate availability may be a challenge. Additionally, the capacity of caffeine to enhance fat utilization during exercise could be of interest for improving health outcomes as it may increase the rate of change in body composition in exercise programs. Genetic factors like CYP1A2 c.-163A\>C, GSTP c.313A\>G and PGC1a c.1444G\>A and C\>T polymorphisms could be associated with the capacity for fat oxidation during exercise. To date, it is unknown if genetics increases fat oxidation and MFO in the same proportion during morning and evening exercise trials in women. For this reason, the aim of the present study was to evaluate the influence of the tCYP1A2, GSTP and PGC1a polymorphisms on the effect of caffeine on fat oxidation and MFO in active and healthy population. The authors hypothesised that genetics would increase fat oxidation and MFO during exercise and this effect would be of similar magnitude at several caffeine doses.
Interventions
DIETARY_SUPPLEMENTAcute caffeine supplementation
To evaluate the influence of the time of the day (i.e., morning vs evening) on the effect of caffeine on maximal fat oxidation in women
Sponsors
Universidad Francisco de Vitoria
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
DOUBLE (Subject, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
18 Years to 40 Years
Healthy volunteers
No
Inclusion criteria
To be non-smokers. To have low caffeine intake (i.e., \< 50 mg of caffeine per day in the previous 2 months) To show no previous history of cardiopulmonary diseases or having suffered musculoskeletal injuries in the previous 6 months.
Exclusion criteria
To have VO2max values below 40 ml/kg/min To be sedentary
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Genotype frequency of CYP1A2 polymorphism | Baseline | Samples shall be obtained by swabbing and scraping of the buccal mucosa by the participant. The c.-163A\>C (rs762551) polymorphism will be used. |
| Genotype frequency of GSTP polymorphism | Baseline | Samples shall be obtained by swabbing and scraping of the buccal mucosa by the participant. The c.1444G\>A (rs8192678) and C\>T (rs17650401) polymorphisms will be used. |
| Genotype frequency of PGC1a polymorphisms | Baseline | Samples shall be obtained by swabbing and scraping of the buccal mucosa by the participant. The c.313A\>G (rs1695) polymorphism will be used. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| FATmax | 2-months | The intensity of exercise that elicits MFO |
| FAT and CHO oxidation | 2-months | Fat and carbohydrates oxidation |
| MFO | 2-months | Maximal fat oxidation during exercise |
| RPE | 2-months | Rate of percevied exertion during exercise |
Countries
Spain
Outcome results
None listed