Embryo Quality
Conditions
Keywords
follicle size, micro RNA, blastocyst culture, embryo quality
Brief summary
In this study, it is aimed to analyze the outcomes of embryos obtained from ovarian follicle with different sizes, and identify the microRNA expression in the spent media of the blastocysts cultures.
Detailed description
In this study, it is aimed to analyze the outcomes of embryos obtained from ovarian follicle with different sizes. By this way, we assume to predict which follicle is able to give the best blastocyst. After the blastocysts are obtained from the different sized follicles, blastocyst cultures are going to be studied for the up and down-regulations of microRNA, besides the correlation of embryo quality with miRNA expression in the spent media.
Interventions
PROCEDUREOocyte pick-up
With the needle, transvaginally USG-guided oocyte pick-up procedure is going to be performed
Sponsors
Acibadem University
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
FEMALE
Age
20 Years to 38 Years
Healthy volunteers
Yes
Inclusion criteria
* Female patients aged between 20-38 who are planned to have IVF treatment * On the day of trigger before the oocyte pick-up, patients having at least 5, and 14 at most follicles exceeding 11mm * Patient having BMI below 30
Exclusion criteria
* PCO patients * Patients having endometriosis * Patients having male factor with severe oligoazoospermia
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Oocyte grading | after each hyper stimulation process of the patients, when the follicle sizes are large enough to pick up which is roughly 10-15 days after the hyper stimulation has started | Morphologic Oocyte grading will be analyzed right after the procedure due to different follicle sizes |
| Day-3 Embryo Grading | Three days after the intracytoplasminc sperm injection(ICSI) procedure | Morphologic Day-3 embryo grading will be analyzed after the procedure due to different follicle sizes |
| Day-5 Embryo Grading | 5 days after the intracytoplasminc sperm injection(ICSI) procedure | Day-5 embryo grading(Gardner classification) will be analyzed after the procedure due to different follicle sizes |
| beta hCG results of the transferred embryos | ten days after the embryo transfer | Clinical pregnancy outcomes |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| micro RNA expression in culture medium | Culture mediums of day 5 embryos will be collected to be studied, after the last embryo is obtained and all will be studied at the end of the recruitment | In the culture medium of blastocysts are going to be analyzed for microRNA levels that are expressed |
Countries
Turkey (Türkiye)
Contacts
Primary ContactNAZLI ALBAYRAK, MD
Backup ContactTURGUT AYDIN, MD
Outcome results
None listed