Sepsis
Conditions
Keywords
Sepsis, monocytes, neutrophils, genetic polymorphism, PDRF
Brief summary
A comprehensive strategy will be used to investigate the relationship and correlation between 4 diagnostically significant markers relevant for early diagnosis and prediction of complications and death in the development of sepsis in children (C-reactive protein, procalcitonin, presepsin and lipopolysaccharide binding protein). For the first time, an attempt will be made to assess the genetic characteristics of the patient's from the point of view of predisposition to the unfavorable development of the sepsis based on the study of polymorphism of a number of genes of the immune system (tumor necrosis factor beta; interleukin 6, 8, 10; lymphotoxin alpha, etc.). Based on the study results, an algorithm to predict the unfavorable course of sepsis in children will be developed using a comprehensive assessment of biochemical and molecular genetic markers.
Detailed description
* analyze biochemical markers and immune status data in sepsis patients and in the comparison group; * assess the state of the cellular immunity, level of pro-inflammatory cytokines, genetic polymorphism of immune response genes in sepsis patients; * carry out a correlation analysis of clinical and laboratory data and immune system among patients of different groups (with and without septic shock, taking into account the outcome); * assess the relationship between the genetic characteristics of the patient's immune system and the severity of the pathological process; * based on the data obtained, prepare instructions for use, which describes an algorithm to predict the unfavorable course of sepsis in children.
Interventions
DIAGNOSTIC_TESTBood leukocyte subsets
Determination of blood leukocyte subpopulations, subpopulation of monocytes and expression CD64 on neutrophils
Sponsors
Belarusian State Medical University
The Republican Research and Practical Center for Epidemiology and Microbiology
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
1 Days to 18 Years
Healthy volunteers
Yes
Inclusion criteria
* age from 1 month to 18 years; * confirmed septic process$ * informed consent.
Exclusion criteria
* age from 18 years; * refuse of patient to participate in the trial; * chronic mental disorders with severe manifestations; * pregnancy/lactation; * intercurrent severe chronic diseases; * HIV, Hepatites B/C; * active tuberculosis; * cachexia of any origin; * malignant neoplasms.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | 1 month | Determination of blood leukocyte subpopulations and their total number in the blood samples (10\^9 cells/l): WBC (leukocytes), CD45+(lymphocytes),CD3+ (T-lymphocytes), CD3- CD16/56+ (NK-cells), CD3+ CD16/56+ (NKT-cells), CD3+ CD4+ (T-helpers), CD3+ CD8+ (T-cytotoxic cells), CD19+ (B-lymphocytes), CD14+ (monocytes): CD14+CD16- (сlassical monocytes), CD14+СD16+(Intermediate monocytes), СD14-СD16+(nonclassical monocytes). Leucocyte count was determined under a microscope in a Goryaev chamber. |
| Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | 1 month | Determination of the relative subpopulations of blood leukocytes (percentage of cells of parent population, %): CD45+ (% leukocytes that are lymphocytes), CD3+ (% lymphocytes that are T-cells), CD3- CD16/56+ (% lymphocytes that are NK-cells), CD3+ CD16/56+ (% T-lymphocytes that are NKT-cells), CD3+ CD4+ (% T-lymphocytes that are T-helpers), CD3+ CD8+ (% T-lymphocytes that are T-cytotoxic cells), CD19+ (% lymphocytes that are B-cells), CD14+ CD16- (% monocytes that are classical), CD14+ CD16+ (% monocytes that are intermediate), CD14- CD16+ (% monocytes that are nonclassical), nCD64+ (% neutrophils that express CD64), mHLA-DR (% monocytes that express human leukocyte antigen-DR (HLA-DR)). Cell samples were counted on a FACSCalibur cytofluorimeter. Data were analyzed using Flowing Software version 2.5.1 or BD FACSDiva 7.0. |
Countries
Belarus
Participant flow
Participants by arm
| Arm | Count |
|---|---|
| Patients With Sepsis on Day 1 Bood leukocyte subsets: Determination of blood leukocyte subpopulations, subpopulation of monocytes and expression CD64 on neutrophils | 50 |
| Patients With Sepsis on Day 7 Bood leukocyte subsets: Determination of blood leukocyte subpopulations, subpopulation of monocytes and expression CD64 on neutrophils | 47 |
| Patients With Severe Bacterial Infection Bood leukocyte subsets: Determination of blood leukocyte subpopulations, subpopulation of monocytes and expression CD64 on neutrophils | 33 |
| Patients With Severe Viral Infection Bood leukocyte subsets: Determination of blood leukocyte subpopulations, subpopulation of monocytes and expression CD64 on neutrophils | 20 |
| Control Group Bood leukocyte subsets: Determination of blood leukocyte subpopulations, subpopulation of monocytes and expression CD64 on neutrophils | 35 |
| Total | 185 |
Baseline characteristics
| Characteristic | Patients With Severe Bacterial Infection | Patients With Severe Viral Infection | Control Group | Total | Patients With Sepsis on Day 1 | Patients With Sepsis on Day 7 |
|---|---|---|---|---|---|---|
| Age, Categorical <=18 years | 33 Participants | 20 Participants | 35 Participants | 185 Participants | 50 Participants | 47 Participants |
| Age, Categorical >=65 years | 0 Participants | 0 Participants | 0 Participants | 0 Participants | 0 Participants | 0 Participants |
| Age, Categorical Between 18 and 65 years | 0 Participants | 0 Participants | 0 Participants | 0 Participants | 0 Participants | 0 Participants |
| Race and Ethnicity Not Collected | — | — | — | 0 Participants | — | — |
| Region of Enrollment Belarus | 33 participants | 20 participants | 35 participants | 185 participants | 50 participants | 47 participants |
| Sex: Female, Male Female | 21 Participants | 14 Participants | 16 Participants | 91 Participants | 20 Participants | 20 Participants |
| Sex: Female, Male Male | 12 Participants | 6 Participants | 19 Participants | 94 Participants | 30 Participants | 27 Participants |
Adverse events
| Event type | EG000 affected / at risk | EG001 affected / at risk | EG002 affected / at risk | EG003 affected / at risk | EG004 affected / at risk |
|---|---|---|---|---|---|
| deaths Total, all-cause mortality | 3 / 50 | 0 / 47 | 0 / 33 | 0 / 20 | 0 / 35 |
| other Total, other adverse events | 0 / 50 | 0 / 47 | 0 / 33 | 0 / 20 | 0 / 35 |
| serious Total, serious adverse events | 3 / 50 | 0 / 47 | 0 / 33 | 0 / 20 | 0 / 35 |
Outcome results
Primary
Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %)
Determination of the relative subpopulations of blood leukocytes (percentage of cells of parent population, %): CD45+ (% leukocytes that are lymphocytes), CD3+ (% lymphocytes that are T-cells), CD3- CD16/56+ (% lymphocytes that are NK-cells), CD3+ CD16/56+ (% T-lymphocytes that are NKT-cells), CD3+ CD4+ (% T-lymphocytes that are T-helpers), CD3+ CD8+ (% T-lymphocytes that are T-cytotoxic cells), CD19+ (% lymphocytes that are B-cells), CD14+ CD16- (% monocytes that are classical), CD14+ CD16+ (% monocytes that are intermediate), CD14- CD16+ (% monocytes that are nonclassical), nCD64+ (% neutrophils that express CD64), mHLA-DR (% monocytes that express human leukocyte antigen-DR (HLA-DR)). Cell samples were counted on a FACSCalibur cytofluorimeter. Data were analyzed using Flowing Software version 2.5.1 or BD FACSDiva 7.0.
Time frame: 1 month
| Arm | Measure | Group | Value (MEAN) |
|---|---|---|---|
| Patients With Sepsis, Day 1 | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % monocytes that are classical | 74.6 percentage of cells of parent population |
| Patients With Sepsis, Day 1 | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % neutrophils that express CD64 | 83.9 percentage of cells of parent population |
| Patients With Sepsis, Day 1 | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % leukocytes that are lymphocytes | 15.2 percentage of cells of parent population |
| Patients With Sepsis, Day 1 | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % T-lymphocytes that are NKT-cells | 1.19 percentage of cells of parent population |
| Patients With Sepsis, Day 1 | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % T-lymphocytes that are T-cytotoxic cells | 20.1 percentage of cells of parent population |
| Patients With Sepsis, Day 1 | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % monocytes that express HLA-DR | 58.98 percentage of cells of parent population |
| Patients With Sepsis, Day 1 | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % monocytes that are nonclassical | 2.03 percentage of cells of parent population |
| Patients With Sepsis, Day 1 | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % lymphocytes that are NK-cells | 5.42 percentage of cells of parent population |
| Patients With Sepsis, Day 1 | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % T-lymphocytes that are T-helpers | 37.6 percentage of cells of parent population |
| Patients With Sepsis, Day 1 | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % lymphocytes that are B-cells | 20.5 percentage of cells of parent population |
| Patients With Sepsis, Day 1 | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % lymphocytes that are T-cells | 67.4 percentage of cells of parent population |
| Patients With Sepsis, Day 1 | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % monocytes that are intermediate | 11.4 percentage of cells of parent population |
| Patients With Sepsis, Day 7 | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % monocytes that express HLA-DR | 88.54 percentage of cells of parent population |
| Patients With Sepsis, Day 7 | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % T-lymphocytes that are T-cytotoxic cells | 24.8 percentage of cells of parent population |
| Patients With Sepsis, Day 7 | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % lymphocytes that are T-cells | 71.5 percentage of cells of parent population |
| Patients With Sepsis, Day 7 | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % lymphocytes that are B-cells | 17.3 percentage of cells of parent population |
| Patients With Sepsis, Day 7 | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % leukocytes that are lymphocytes | 30.3 percentage of cells of parent population |
| Patients With Sepsis, Day 7 | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % neutrophils that express CD64 | 5.55 percentage of cells of parent population |
| Patients With Sepsis, Day 7 | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % lymphocytes that are NK-cells | 7.4 percentage of cells of parent population |
| Patients With Sepsis, Day 7 | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % monocytes that are classical | 81.2 percentage of cells of parent population |
| Patients With Sepsis, Day 7 | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % monocytes that are nonclassical | 1.15 percentage of cells of parent population |
| Patients With Sepsis, Day 7 | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % T-lymphocytes that are NKT-cells | 2.2 percentage of cells of parent population |
| Patients With Sepsis, Day 7 | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % monocytes that are intermediate | 7.02 percentage of cells of parent population |
| Patients With Sepsis, Day 7 | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % T-lymphocytes that are T-helpers | 41.4 percentage of cells of parent population |
| Patients With Severe Bacterial Infection | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % neutrophils that express CD64 | 81.4 percentage of cells of parent population |
| Patients With Severe Bacterial Infection | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % leukocytes that are lymphocytes | 15.7 percentage of cells of parent population |
| Patients With Severe Bacterial Infection | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % lymphocytes that are T-cells | 62.9 percentage of cells of parent population |
| Patients With Severe Bacterial Infection | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % lymphocytes that are NK-cells | 4.5 percentage of cells of parent population |
| Patients With Severe Bacterial Infection | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % T-lymphocytes that are NKT-cells | 1.13 percentage of cells of parent population |
| Patients With Severe Bacterial Infection | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % T-lymphocytes that are T-helpers | 36.8 percentage of cells of parent population |
| Patients With Severe Bacterial Infection | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % T-lymphocytes that are T-cytotoxic cells | 19.1 percentage of cells of parent population |
| Patients With Severe Bacterial Infection | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % lymphocytes that are B-cells | 27.4 percentage of cells of parent population |
| Patients With Severe Bacterial Infection | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % monocytes that are classical | 83.0 percentage of cells of parent population |
| Patients With Severe Bacterial Infection | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % monocytes that are intermediate | 6.28 percentage of cells of parent population |
| Patients With Severe Bacterial Infection | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % monocytes that are nonclassical | 2.16 percentage of cells of parent population |
| Patients With Severe Bacterial Infection | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % monocytes that express HLA-DR | 62.63 percentage of cells of parent population |
| Patients With Severe Viral Infection | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % T-lymphocytes that are T-cytotoxic cells | 22.46 percentage of cells of parent population |
| Patients With Severe Viral Infection | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % leukocytes that are lymphocytes | 24.9 percentage of cells of parent population |
| Patients With Severe Viral Infection | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % monocytes that are classical | 78.0 percentage of cells of parent population |
| Patients With Severe Viral Infection | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % T-lymphocytes that are T-helpers | 39.8 percentage of cells of parent population |
| Patients With Severe Viral Infection | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % monocytes that are intermediate | 12.6 percentage of cells of parent population |
| Patients With Severe Viral Infection | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % T-lymphocytes that are NKT-cells | 3.4 percentage of cells of parent population |
| Patients With Severe Viral Infection | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % monocytes that express HLA-DR | 86.4 percentage of cells of parent population |
| Patients With Severe Viral Infection | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % monocytes that are nonclassical | 3.5 percentage of cells of parent population |
| Patients With Severe Viral Infection | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % lymphocytes that are NK-cells | 13.35 percentage of cells of parent population |
| Patients With Severe Viral Infection | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % lymphocytes that are T-cells | 69.3 percentage of cells of parent population |
| Patients With Severe Viral Infection | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % neutrophils that express CD64 | 0.35 percentage of cells of parent population |
| Patients With Severe Viral Infection | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % lymphocytes that are B-cells | 14.18 percentage of cells of parent population |
| Control Group | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % T-lymphocytes that are T-cytotoxic cells | 22.9 percentage of cells of parent population |
| Control Group | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % monocytes that are nonclassical | 1.1 percentage of cells of parent population |
| Control Group | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % leukocytes that are lymphocytes | 44.5 percentage of cells of parent population |
| Control Group | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % monocytes that are classical | 82.8 percentage of cells of parent population |
| Control Group | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % lymphocytes that are T-cells | 71.5 percentage of cells of parent population |
| Control Group | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % T-lymphocytes that are NKT-cells | 3.4 percentage of cells of parent population |
| Control Group | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % monocytes that express HLA-DR | 67.0 percentage of cells of parent population |
| Control Group | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % T-lymphocytes that are T-helpers | 34.8 percentage of cells of parent population |
| Control Group | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % monocytes that are intermediate | 6.1 percentage of cells of parent population |
| Control Group | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % lymphocytes that are B-cells | 13.5 percentage of cells of parent population |
| Control Group | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % lymphocytes that are NK-cells | 11.6 percentage of cells of parent population |
| Control Group | Blood Leukocyte Subpopulations: Flow Cytometry Measure (Percentage of Cells of Parent Population, %) | % neutrophils that express CD64 | 0.7 percentage of cells of parent population |
p-value: <0.05Wilcoxon (Mann-Whitney)
p-value: <0.05Wilcoxon (Mann-Whitney)
p-value: <0.02Wilcoxon (Mann-Whitney)
Primary
Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes
Determination of blood leukocyte subpopulations and their total number in the blood samples (10\^9 cells/l): WBC (leukocytes), CD45+(lymphocytes),CD3+ (T-lymphocytes), CD3- CD16/56+ (NK-cells), CD3+ CD16/56+ (NKT-cells), CD3+ CD4+ (T-helpers), CD3+ CD8+ (T-cytotoxic cells), CD19+ (B-lymphocytes), CD14+ (monocytes): CD14+CD16- (сlassical monocytes), CD14+СD16+(Intermediate monocytes), СD14-СD16+(nonclassical monocytes). Leucocyte count was determined under a microscope in a Goryaev chamber.
Time frame: 1 month
| Arm | Measure | Group | Value (MEAN) |
|---|---|---|---|
| Patients With Sepsis, Day 1 | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | сlassical monocytes,10^9 cells/l | 0.66 10^9 cells/l |
| Patients With Sepsis, Day 1 | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | T-helpers,10^9 cells/l | 0.65 10^9 cells/l |
| Patients With Sepsis, Day 1 | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | lymphocyte, 10^9 cells/l | 1.83 10^9 cells/l |
| Patients With Sepsis, Day 1 | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | Intermediate monocytes,10^9 cells/l | 0.09 10^9 cells/l |
| Patients With Sepsis, Day 1 | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | T-cytotoxic,10^9 cells/l | 0.40 10^9 cells/l |
| Patients With Sepsis, Day 1 | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | T-lymphocytes,10^9 cells/l | 1.28 10^9 cells/l |
| Patients With Sepsis, Day 1 | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | B-lymphocytes,10^9 cells/l | 0.36 10^9 cells/l |
| Patients With Sepsis, Day 1 | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | monocytes,10^9 cells/l | 1.04 10^9 cells/l |
| Patients With Sepsis, Day 1 | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | nonclassical monocytes,10^9 cells/l | 0.014 10^9 cells/l |
| Patients With Sepsis, Day 1 | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | NK-cells,10^9 cells/l | 0.10 10^9 cells/l |
| Patients With Sepsis, Day 1 | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | NKT-cells,10^9 cells/l | 0.020 10^9 cells/l |
| Patients With Sepsis, Day 1 | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | WBC, 10^9 cells/l | 16.0 10^9 cells/l |
| Patients With Sepsis, Day 7 | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | T-lymphocytes,10^9 cells/l | 2.0 10^9 cells/l |
| Patients With Sepsis, Day 7 | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | Intermediate monocytes,10^9 cells/l | 0.04 10^9 cells/l |
| Patients With Sepsis, Day 7 | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | сlassical monocytes,10^9 cells/l | 0.45 10^9 cells/l |
| Patients With Sepsis, Day 7 | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | T-helpers,10^9 cells/l | 1.0 10^9 cells/l |
| Patients With Sepsis, Day 7 | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | nonclassical monocytes,10^9 cells/l | 0.008 10^9 cells/l |
| Patients With Sepsis, Day 7 | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | WBC, 10^9 cells/l | 11.2 10^9 cells/l |
| Patients With Sepsis, Day 7 | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | B-lymphocytes,10^9 cells/l | 0.54 10^9 cells/l |
| Patients With Sepsis, Day 7 | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | NKT-cells,10^9 cells/l | 0.050 10^9 cells/l |
| Patients With Sepsis, Day 7 | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | T-cytotoxic,10^9 cells/l | 0.69 10^9 cells/l |
| Patients With Sepsis, Day 7 | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | NK-cells,10^9 cells/l | 0.14 10^9 cells/l |
| Patients With Sepsis, Day 7 | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | lymphocyte, 10^9 cells/l | 3.10 10^9 cells/l |
| Patients With Sepsis, Day 7 | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | monocytes,10^9 cells/l | 0.78 10^9 cells/l |
| Patients With Severe Bacterial Infection | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | сlassical monocytes,10^9 cells/l | 0.76 10^9 cells/l |
| Patients With Severe Bacterial Infection | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | B-lymphocytes,10^9 cells/l | 0.58 10^9 cells/l |
| Patients With Severe Bacterial Infection | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | lymphocyte, 10^9 cells/l | 1.93 10^9 cells/l |
| Patients With Severe Bacterial Infection | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | monocytes,10^9 cells/l | 0.87 10^9 cells/l |
| Patients With Severe Bacterial Infection | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | T-lymphocytes,10^9 cells/l | 1.24 10^9 cells/l |
| Patients With Severe Bacterial Infection | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | WBC, 10^9 cells/l | 11.9 10^9 cells/l |
| Patients With Severe Bacterial Infection | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | NK-cells,10^9 cells/l | 0.10 10^9 cells/l |
| Patients With Severe Bacterial Infection | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | Intermediate monocytes,10^9 cells/l | 0.06 10^9 cells/l |
| Patients With Severe Bacterial Infection | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | NKT-cells,10^9 cells/l | 0.025 10^9 cells/l |
| Patients With Severe Bacterial Infection | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | nonclassical monocytes,10^9 cells/l | 0.015 10^9 cells/l |
| Patients With Severe Bacterial Infection | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | T-helpers,10^9 cells/l | 0.73 10^9 cells/l |
| Patients With Severe Bacterial Infection | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | T-cytotoxic,10^9 cells/l | 0.39 10^9 cells/l |
| Patients With Severe Viral Infection | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | nonclassical monocytes,10^9 cells/l | 0.011 10^9 cells/l |
| Patients With Severe Viral Infection | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | WBC, 10^9 cells/l | 4.9 10^9 cells/l |
| Patients With Severe Viral Infection | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | lymphocyte, 10^9 cells/l | 1.14 10^9 cells/l |
| Patients With Severe Viral Infection | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | T-lymphocytes,10^9 cells/l | 0.81 10^9 cells/l |
| Patients With Severe Viral Infection | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | NK-cells,10^9 cells/l | 0.15 10^9 cells/l |
| Patients With Severe Viral Infection | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | NKT-cells,10^9 cells/l | 0.038 10^9 cells/l |
| Patients With Severe Viral Infection | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | T-helpers,10^9 cells/l | 0.40 10^9 cells/l |
| Patients With Severe Viral Infection | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | T-cytotoxic,10^9 cells/l | 0.30 10^9 cells/l |
| Patients With Severe Viral Infection | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | B-lymphocytes,10^9 cells/l | 0.16 10^9 cells/l |
| Patients With Severe Viral Infection | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | monocytes,10^9 cells/l | 0.4 10^9 cells/l |
| Patients With Severe Viral Infection | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | сlassical monocytes,10^9 cells/l | 0.28 10^9 cells/l |
| Patients With Severe Viral Infection | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | Intermediate monocytes,10^9 cells/l | 0.05 10^9 cells/l |
| Control Group | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | T-cytotoxic,10^9 cells/l | 0.74 10^9 cells/l |
| Control Group | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | WBC, 10^9 cells/l | 6.9 10^9 cells/l |
| Control Group | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | сlassical monocytes,10^9 cells/l | 0.38 10^9 cells/l |
| Control Group | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | T-helpers,10^9 cells/l | 1.19 10^9 cells/l |
| Control Group | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | NKT-cells,10^9 cells/l | 0.083 10^9 cells/l |
| Control Group | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | NK-cells,10^9 cells/l | 0.36 10^9 cells/l |
| Control Group | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | nonclassical monocytes,10^9 cells/l | 0.004 10^9 cells/l |
| Control Group | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | Intermediate monocytes,10^9 cells/l | 0.027 10^9 cells/l |
| Control Group | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | T-lymphocytes,10^9 cells/l | 2.1 10^9 cells/l |
| Control Group | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | lymphocyte, 10^9 cells/l | 3.24 10^9 cells/l |
| Control Group | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | monocytes,10^9 cells/l | 0.46 10^9 cells/l |
| Control Group | Blood Leukocyte Subpopulations: the Absolute Numbers of Leukocytes of Specific Phenotypes | B-lymphocytes,10^9 cells/l | 0.43 10^9 cells/l |
p-value: <0.05Wilcoxon (Mann-Whitney)