Infertility, IVF
Conditions
Keywords
Natural IVF, Follicular Fluid, Oxidation-reduction potential, Gonadotrophin, Lifestyle
Brief summary
The goal of this observational study is to understand the processes in the growing follicles development in more detail and to improve In Vitro Fertilisation (IVF) treatments. This involves measuring laboratory values in the blood, in the follicular fluid of the woman and in the sperm of the man as well as questionnaires concerning lifestyle factors. These values are then linked to the success of the fertility treatment to find out which of these values increase the success of the IVF treatment. Furthermore, the investigators want to investigate if there are differences between IVF treatments with or without hormonal stimulation of the ovaries.
Detailed description
This is a one-site prospective exploratory study at the University Clinic for Gynaecological Endocrinology and Reproductive Medicine in Bern. Data of influencing factors on IVF success in women and men will be collected in two types of IVF treatment, i.e. with or without hormonal stimulation of the ovaries. The investigators want to * compare the follicular fluid values between the two IVF treatment groups. * assess the associations and predictive power of follicular fluid / blood and sperm parameters for success of the IVF treatment. * compare the IVF success in the two treatment groups. The study aims to enrol approximately 200 couples. The data will be collected by the physicians and added to the REDcap study registry. Access to the total data set is only permitted for the principal investigator.
Interventions
PROCEDURENatural cycle In Vitro Fertilisation (IVF)
In Vitro Fertilisation (IVF) without hormonal stimulation
DIAGNOSTIC_TESTBlood samples for analysis
Test hormone levels in serum
DIAGNOSTIC_TESTFollicular fluid for analysis
Test hormone levels in follicular fluid Test Oxidation-reduction potential
DIAGNOSTIC_TESTSperm samples for analysis
Oxidation-reduction potential
PROCEDUREConventional In Vitro Fertilisation (IVF)
In Vitro Fertilisation (IVF) with conventional hormonal stimulation
Sponsors
Janna Pape
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 42 Years
Healthy volunteers
No
Inclusion criteria
* Willing to participate * Written consent * Couples undergoing In Vitro Fertilisation (natural cycle or conventional IVF)
Exclusion criteria
* Not willing to participate * Missing consent * Female of the couple does not want to participate or missing consent * Language barrier * Medical freezing * Social freezing * Thawed cycles * Genetic disorders * Preimplantation genetic testing (PGT) * Medication for chronic condition * Surgery in the last 4 weeks * Significant trauma in the last 90 days * Severe reduced sperm quality * Kryosperm * Poor responder or expected poor response
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Blastocyst rate per oocyte | 5 days after fertilization | Number of blastocysts out of fertilized oocytes observed 5 days after fertilization |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Cleavage rate per oocyte | 2 days after fertilization | Number of cleavage embryos out of fertilized oocytes observed 2 days after fertilization |
| Morphologic evaluation of blastocyst | 5 days after fertilization | Morphologic evaluation according to the Gardner score (Gardner and Schoolcraft, 1999) |
| Number of mature oocytes | 4-6 hours after punction | Number of mature oocytes from punctured follicles |
| Clinical pregnancy rate | 5 weeks after embryo transfer | Clinical pregnancy confirmed by ultrasound, 5 weeks after embryo transfer. |
| Live birth rate | 9 months after embryo transfer | Birth of a living baby nine months after embryo transfer |
| Implantation rate | 10-12 days after embryo transfer | Endocrine pregnancy by measuring hCG in blood sample on day 10-12 after embryo transfer |
Countries
Switzerland
Contacts
Primary ContactJanna Pape, MD
Backup ContactMichael von Wolff, Prof. Dr.
Outcome results
None listed