Normal-weight Obesity, Obesity
Conditions
Brief summary
Exercise acutely increases gut permeability and inflammation, even in healthy populations. However, whether this response differs in groups at-risk for CVD that present with low-grade inflammation (e.g., normal-weight obesity) has yet to be examined. The investigators aim to measure serum indicators of gut permeability in those with normal-weight obesity pre- and post-short, intense exercise and sustained, moderate exercise
Detailed description
The investigator's overall goal is to examine how indicators of gut permeability change in response to exercise in individuals with normal-weight obesity (i.e., normal body mass index \[BMI\] and high body fat percent) relative to those with a normal BMI and low body fat percent and those with a BMI in the obesity range and high body fat percent. The investigators will examine indicators of gut permeability (specified in primary outcomes) in response to a short, intense bout of exercise (i.e., VO2 max test on a cycle ergometer) and sustained, moderate bout of exercise (i.e., 45 minutes at 65% measured VO2 max on a cycle ergometer). Additionally, the investigators will measure basic anthropometrics, blood lipids and glucose, and body composition with DXA.
Interventions
OTHERExercise tests (VO2 max test and 45-minute exercise session at 65% VO2 max)
All participants will complete two exercise sessions: 1. VO2 max test on a cycle ergometer 2. Moderate exercise session (45 minutes at 65% measured VO2 max)
Sponsors
Oklahoma State University
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
PREVENTION
Masking
NONE
Intervention model description
Three study groups (n=10/ group): 1. Normal weight and low body fat percent (BMI 18.5-24.9 kg/m2, body fat percent \< 25% in males or \< 35% in females), 2. Normal-weight obesity (BMI 18.5-24.9 kg/m2, body fat percent \> 25% in males or \> 35% in female) 3. Overt obesity and high body fat percent (BMI \> 30 kg/m2, body fat percent \> 25% in males or \> 35% in females)
Eligibility
Sex/Gender
ALL
Age
18 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
Control group: normal BMI, body fat percentage \< 25% (male) or \< 35% (female) Normal-weight obesity: normal BMI, body fat percentage \> 25% (male) or \> 35% (female) Overt obesity: BMI in obesity range, body fat percentage \> 25% (male) or \> 35% (female)
Exclusion criteria
* Presence of pacemaker * Pregnant * Postmenopausal status * History of chronically using of tobacco products, illicit drugs, anti-inflammatory drugs (e.g., NSAIDs), lipid lowering drugs * Recent use of antibiotics (\< 6 weeks) and NSAIDs (\< 3 days) * Established cardiometabolic disease (e.g., cardiovascular disease, type 2 diabetes) diseases inflammatory in nature (e.g., rheumatoid arthritis, inflammatory bowel disease), and/or diagnosed irritable bowel syndrome.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Serum concentrations of FABP2 following short, intense exercise | Approximately 8 months | The investigators will examine peak and absolute change in this indicator of gut permeability after the VO2 max test on a cycle ergometer. |
| Serum concentrations of IL-6 following sustained, moderate exercise | Approximately 8 months | The investigators will examine peak and absolute change in this inflammatory marker after 45-minutes of exercise at 65% VO2 max on a cycle ergometer. |
| Serum concentrations of FABP2 following sustained, moderate exercise | Approximately 8 months | The investigators will examine peak and absolute change in this indicator of gut permeability after 45-minutes of exercise at 65% VO2 max on a cycle ergometer. |
| Serum concentrations of sCD14 following short, intense exercise | Approximately 8 months | The investigators will examine peak and absolute change in this indicator of gut permeability after the VO2 max test on a cycle ergometer. |
| Serum concentrations of LBP following short, intense exercise | Approximately 8 months | The investigators will examine peak and absolute change in this indicator of gut permeability after the VO2 max test on a cycle ergometer. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Respiratory exchange ratio (RER) during VO2 max test | Approximately 8 months | The investigators will compare RER dynamics during the short, intense exercise session across study groups. |
| VO2 Max | Approximately 8 months | The investigators will compare VO2 max after the short, intense exercise session across study groups. |
| Maximum workload during exercise sessions | Approximately 8 months | The investigators will compare workload dynamics in watts during the short, intense and moderate, sustained exercise session across study groups. |
| Heart rate dynamics during exercise sessions | Approximately 8 months | The investigators will compare heart rate dynamics during the short, intense and moderate, sustained exercise session across study groups. |
Other
| Measure | Time frame | Description |
|---|---|---|
| Absolute body fat assessment with dual-energy X-ray absorptiometry (DXA) | Approximately 8 months | The investigators will perform DXA scans on all participants to assess absolute body fat in kilograms. |
| Lipid panel with Abbott Piccolo Xpress Clinical Chemistry Analyzer | Approximately 8 months | The investigators will perform a lipid panel on all participants. |
| Absolute lean mass assessment with dual-energy X-ray absorptiometry (DXA) | Approximately 8 months | The investigators will perform DXA scans on all participants to assess absolute lean mass in kilograms. |
| Absolute visceral fat mass assessment with dual-energy X-ray absorptiometry (DXA) | Approximately 8 months | The investigators will perform DXA scans on all participants to assess absolute visceral mass in grams. |
| Body fat percent assessment with dual-energy X-ray absorptiometry (DXA). | Approximately 8 months | The investigators will perform DXA scans on all participants to assess body fat percent. |
| Lean mass percent assessment with dual-energy X-ray absorptiometry (DXA) | Approximately 8 months | The investigators will perform DXA scans on all participants to assess lean mass percent. |
Countries
United States
Contacts
Primary ContactBryant H Keirns, MS
Backup ContactSam R Emerson, PhD
Outcome results
None listed