Azoospermia, Infertility, Male, Reproductive Disorder
Conditions
Brief summary
Normal embryonic development relies on the correct transmission of genetic information, and sperm DNA plays a crucial part in this process. Causes of poor sperm DNA integrity include unhealthy lifestyles such as smoking and exposure to gonadotoxins, as well as, obesity, varicoceles, infections, advanced paternal age and systemic disorders. An increase in DNA fragmentation in sperm has been linked to lower fertilisation rate, poorer quality embryos, lower pregnancy rate, and high miscarriages rate. The best way for sperm selection and processing in assisted reproductive technologies (ART) should be noninvasive and cost-effective. It should also make it possible to identify high-quality spermatozoa and produce more favorable results in terms of pregnancy and live birth rates.7 Meanwhile, the microfluidic sperm separation technology is a less expensive and less invasive alternative. This method allows for the selection of motile sperm that have a normal morphology, low levels of reactive oxygen species (ROS), and low DFI
Interventions
None listed
Sponsors
Clinique Ovo
Study design
Observational model
COHORT
Time perspective
RETROSPECTIVE
Eligibility
Sex/Gender
MALE
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* Men with high DNA fragmentation (\>20%) undergoing TESA-ICSI or Zymot-ICSI
Exclusion criteria
* Spouse with advanced maternal age (\> 40 years) * Egg donation cycle
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Clinical Pregnancy Rate | approximately 8 weeks | Compare clinical pregnancy rate in couples with high Sperm DNA fragmentation |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Number of utilizable blastocysts obtained | Approximately 20 days | Compare the number of utilizable embryos obtained in couples using TESA-ICSI vs Zymot-ICSI |
Countries
Canada
Outcome results
None listed