Multiple Myeloma
Conditions
Brief summary
Multiple myeloma (MM) is a haematological malignancy characterized by the accumulation of proliferating antibody producing plasma cells in the bone marrow. In the last few years many studies have emphasized the biological complexity and heterogeneity of MM as a result of both genetic and epigenetic aberrations. Che-1/AATF (Che-1) is a transcriptional cofactor involved in cell cycle regulation, apoptosis, DNA damage and stress response. it can be hypothesized that Che-1 might contribute to the establishment of the MM malignant phenotype by sustaining global transcription through its ability to modulate chromatin accessibility. The prospective study on MM human samples aims to understand the involvement of Che-1 in the transcriptional regulation and chromatin remodeling during the pathology. To this goal the main objectives are: * Characterization of Che-1's role in chromatin remodeling and global gene expression * Study in vivo and in vitro of Che-1 involvement in MM tumorigenesis * Evaluation of Che-1 as a possible therapeutic target
Interventions
OTHERATAC-seq and ChIP-seq
CD138+ cells will be isolated from the bone marrow of MM patients and will be subjected to ATAC-seq and ChIP-seq analyses, by useing specific antibodies, to better understand the involvment of Che-1 in MM tumorogenesis.
Sponsors
Regina Elena Cancer Institute
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Healthy volunteers
No
Inclusion criteria
* MM Patients
Exclusion criteria
* No
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Che-1 involvement in MM tumorigenesis | About 2 years | Characterization of Che-1's role in chromatin remodeling and global gene expression |
Countries
Italy
Contacts
Primary ContactMaurizio fanciulli, PhD
Outcome results
None listed