Adult Healthy Volunteers
Conditions
Keywords
Olive oil, inflammation, gut microbiota, Mediterranean Diet
Brief summary
This research project, approved and funded, is part of a larger project, entitled Healthy Soil for Food, which completes several work packages, one of which is a clinical research submitted to Ethics Committee entitled O-LIVE: Impact of olive oil Portuguese in biomarkers of healthy volunteers. The main objective of this work is to evaluate the impact of ingestion of an olive oil rich in polyphenols on clinical biomarkers and parameters, such as anthropometric measurements, on inflammatory gene expression regulation and on the composition of the intestinal microbiota.
Detailed description
Olive oil is an ancient product that is crucial for social cohesion and for local economies in the Mediterranean region, particularly in Portugal. There is ample evidence on the protective effect of olive oil in cardiovascular disease, diabetes, oncological diseases and neurological degeneration. Furthermore, olive oil has been shown to increase the rate of oxidation, inhibiting lipogenesis and stimulating lipolysis, in addition to affecting the expression of receptors involved in weight regulation and fat use. These health benefits of olive oil derive from its specific nutritional composition, with monounsaturated fatty acids, polyphenols and phenolic compounds, which act on metabolism, modulation of inflammatory responses and gene expression. But the composition of olive oil can vary considerably depending on the type of cultivation of olive trees, soil management practices, as well as the extraction and technological processes involved in olive oil production. In this work the investigators will use an olive oil rich in polyphenols (produced in a sustainable and local manner) and evaluate its effect on several important clinical parameters involved in chronic diseases. The investigators anticipate that the results of this work will help support the relationship between consuming higher polyphenol olive oil and improved overall health.
Interventions
OTHEROlive-oil
Participants will add to their usual diet a daily intake of 30 mL of an olive oil rich in polyphenols for 3-months (100 days)
Sponsors
Universidade Católica Portuguesa
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
OTHER
Masking
NONE
Intervention model description
This project consists of a quasi-experimental clinical study whose target population is healthy, voluntary adults. Participants will be recruited among men and women, aged 18 years or over, healthy, who belong to the community of the University (Portuguese Catholic University - Porto campus) and who do not meet any of the exclusion criteria. Individuals will be invited to participate voluntarily in this study, receiving detailed information about the investigation, in order to allow an informed, informed and free decision. Individuals who agree to participate sign an informed consent.
Eligibility
Sex/Gender
ALL
Age
18 Years to 55 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy volunteers
Exclusion criteria
* Current active infections * Medications (Antidepressant medication, Antibiotics, anti-inflammatory, Angiotensin Converting Enzyme inhibitors, insulin and oral anti-diabetic medication) * Dietary supplements (minerals and oligoelements, antioxidants, n-3 polyunsaturated fatty acids, n-6 polyunsaturated fatty acids, n-9 fatty acids) * Presence of ant chronic/metabolic diseases: * Diabetes * Cancer * Inflammatory diseases
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| glycated hemoglobin A1c serum levels | Day 1 | drop of blood will be analysed for biochemical parameters with point of care technology using a finger prick and analysed for levels glycated hemoglobin (ha1c) |
| Lipid profile serum level | Day 1 | A drop of blood will be analysed for biochemical parameters with point of care technology using a finger prick and analysed for total cholesterol, HDL, LDL and triacylglycerols |
| C-reactive Protein serum level | Day 1 | A drop of blood will be analysed for biochemical parameters with point of care technology using a finger prick and analysed for PCR levels |
| Body weight | Day 1 | Body weight will be assessed using a scale |
| Bacterial populations in fecal cultures | Day 1 | Bacterial populations in fecal cultures will be analysed by microbial DNA extraction and quantitative real-time polymerase-chain reaction (PCR) using specific primers. |
| Inflammatory gene expression | Day 1 | Saliva sample will also be collected for subsequent DNA extraction and application of quantitative real-time PCR to determine the expression of inflammatory genes. |
| Body composition (% of fat mass and % of non-fat mass) | Day 1 | Body composition will be assessed using Body Impedance Analysis (body composition analyser) |
| Adhesion to mediterranean diet | Day 1 | PREDIMED (PREvencion con Diet MEDiterranea) is a brief 14-item tool able to capture a strong monotonic inverse association between adherence to a good quality dietary pattern (Mediterranean diet) and obesity indexes in a population of adults (doi:10.1371/journal.pone.0043134) |
Countries
Portugal
Contacts
Primary ContactMarta Correia, PhD
Outcome results
None listed