Anorexia Nervosa, Healthy Volunteer
Conditions
Keywords
anorexia nervosa, microbiota, gut-brain-axis, immunology (IgA)
Brief summary
Anorexia nervosa is characterized by intestinal dysbiosis, related to the feeding behavior impairment presented by these patients. Pathophysiologic hypotheses are involving the trouble of the microbiota-gut-brain axis in the field of mental diseases.
Detailed description
Anorexia nervosa is characterized by intestinal dysbiosis, related to the feeding behavior impairment presented by these patients. Here we aim to characterize the IgA-coated fraction of bacteria from the intestinal microbiota, linked to the regulation of the mucosal immune system and the epithelium physiology. Abnormalities of this microbiota are reported in inflammatory bowel disease and are associated to immune trouble. As food intake plays a key role in shaping microbiota, we aim to correlate data from IgA microbiota to food intake specificity, and to anorexia nervosa phenotypes.
Interventions
OTHERStool sample
Purification by affinity of the IgA coated bacteria from a fecal solution. Genomic analysis of the bacteria for diversity and richness
OTHERFood intake evaluation
Measure (by survey) of food group intake for : lipids /proteins / fibers / carbohydrates
Sponsors
Institut National de Recherche pour l'Agriculture, l'Alimentation et l'Environnement
Laboratory Physiopathologie et biotherapies des infections muqueuses (GIMAP)
Centre Hospitalier Universitaire de Saint Etienne
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
FEMALE
Age
18 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
* female subject, adult, affiliated to a social security system, under the care of the eating disorder department of university hospital of st Etienne * presenting a diagnosis of pure restrictive anorexia nervosa. For acute subgroup : BMI between 11 and 17.5 kg/m2, patient with a first episode of restrictive anorexia nervosa or with hyperphagic episodes. For the chronic subgroup: BMI between 11 and 17.5 kg/m2, disease evolving for more than 5 years. For Healthy volunteer: BMI between 20 and 24 kg/m², stable weight for the last 3 months, no eating disorder or history of eating disorder
Exclusion criteria
* patient does not understand French. * laxative treatment in progress or stopped for less than 15 days, * antibiotic treatment in the two months preceding inclusion, * pre-pro or symbiotic treatment in progress or in the month preceding inclusion, * patient with one of the following comorbid disorders: celiac disease, chronic inflammatory bowel disease, lactose intolerance, unstabilized hyperthyroidism, unstabilized diabetes, * subject under legal protective measures.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Total microbiotic profile | 1 week after inclusion | abundance and diversity parameters of the IgA microbiota fraction of the intestinal microbiota obtained by 16s rDNA analysis of stool suspension samples from acute anorexia nervosa, chronic anorexia nervosa and control subjects. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| ELISA assay of fecal immunoglobulins | 1 week after inclusion | ELISA determination of immunoglobulin A and immunoglobulin M in the stool of acute anorexia nervosa, chronic anorexia nervosa and control subjects. |
| Microbiotic profile of total intestinal microbiota | 1 week after inclusion | Total microbiotic profile (abundance and diversity) of the intestinal microbiota obtained by 16s rDNA analysis of stool suspension samples from acute anorexia nervosa, chronic anorexia nervosa and control subjects. |
| Food intake characteristics | 1 week after inclusion | Qualitative and quantitative assessment of dietary intake by a dietary survey of acute anorexia nervosa, chronic anorexia nervosa and control subjects. |
Countries
France
Outcome results
None listed