Biomarkers
Conditions
Keywords
Food biomarker, Whole grain, Chinese population
Brief summary
The goal of this food intervention study is to screen/validate the whole grain food intake biomarker in health Chinese's population.
Detailed description
Although increased consumption of whole grains (WGs) is associated with improved health outcomes, the dietary intake assessment and health mechanisms for WGs or WG-containing food remain unresolved. In addition, information regarding its consumption and health effects in Chinese people is scarce. Biomarkers of WGs intake would allow a more accurate assessment of its consumption in nutrition studies. The investigators want to perform a crossover study where participants were subjected to a WG-wheat flour or a refine wheat-flour Chinese diet. Using a metabolomics approach, the investigators aimed to identify the WG-specific metabolites present in plasma/urine after consumption WG or WG foods, including new candidate biomarkers of WG intake.
Interventions
OTHERwhole grain-wheat flour food+standardized meals
whole grain food made by 50 g whole grain wheat flour
OTHERrefined wheat flour food+standardized meals
refined grain food made by 50 g refined wheat flour
Sponsors
Peking University
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
NONE
Intervention model description
Randomized Crossover Assignment
Eligibility
Sex/Gender
ALL
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy men and women * age 18-40 years * BMI 18.5-23.9 kg/m2.
Exclusion criteria
* pregnancy or breastfeeding * vegetarian; * smokers; * acute and chronic diseases; * allergic to wheat; * frequent nutrients supplement use; * medication use of antibiotics within 1-month; * more than 3 kg weight change within 3-month; * unwillingness to follow dietary restrictions;
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Changes in metabolite profiles present in blood plasma and urine before the dietary intervention and in kinetics over 24 hours. | Baseline and 0-24 hours after intervention food intake | Metabolite profiles analyzed using a non-targeted metabolomics approach and targeted metabolomics approach with a Orbitrap Mass Analyzer. Blood plasma samples collected at time 0 hours, 2 hours, 4 hours, and 24 hours. Urine fractions collected at 0-2 hours, 2-4 hours, 4-6 hours, 6-9 hours, 9-12 hours, 12-24 hours and 24-48 hours. Identification of biomarkers of acute intake of the foods of interest through the comparison of specific metabolomes after single dose of WG food or refined grain food in Chinese population. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| The influence of gut flora on whole grain markers | after the administration of the test meal | Gut flora abundance and metabolites level correlation analysis |
Countries
China
Outcome results
None listed