Appetitive Behavior, Obesity
Conditions
Brief summary
The present study aims to evaluate in adults with normal weight and with overweight or obesity who live in the city of La Plata the circulating levels of various hormones that regulate appetite and the gene expression of their receptors in peripheral blood mononuclear cells before and after food intake.
Detailed description
the investigators plan a single test meal study in males and females with normal weight (NW) or overweight/obesity (OW/OB) assessing whether plasma appetite regulatory hormones levels are associated with different metabolic and anthropometric variables as well as with appetite sensations.
Interventions
DIETARY_SUPPLEMENTfood (breakfast)
The breakfast composition is 56 kcal% carbohydrates, 12 kcal% protein and 34 kcal% lipids and comprises yogurt, cereals and almonds
Sponsors
Instituto de Desarrollo e Investigaciones Pediátricas Prof. Dr. Fernando E. Viteri
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
PREVENTION
Masking
NONE
Intervention model description
Participants will be given a test meal (breakfast) that provides 20% of the individual's daily energy requirement. The breakfast composition is 56 kcal% carbohydrates, 12 kcal% protein and 34 kcal% lipids and comprises yogurt, cereals and almonds. Participants will be asked to consume the entire breakfast in less than 15 min and to complete a visual analog scale (VAS) questionnaire every 15 min. Blood sampling will be repeated at 60 min after starting breakfast (postprandial).
Eligibility
Sex/Gender
ALL
Age
18 Years to 50 Years
Healthy volunteers
Yes
Inclusion criteria
* adult individuals body mass index\> 18.5 kg∙m-2
Exclusion criteria
* chronic medical illness, personal history of diabetes, history of medical condition, or medication related to obesity or diabetes risk status. Pregnancy
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| plasma ghrelin concentration | pre -prandial | ghrelin concentration (fmol/ml) |
| plasma leptin concentration | pre -prandial | leptin concentration (ug/ml) |
| plasma glucagon-like peptide 1 concentration | pre -prandial | glucagon-like peptide 1 concentration (ug/ml) |
| plasma liver antimicrobial peptide 2 concentration | pre -prandial | liver antimicrobial peptide 2 concentration (pmol/ml) |
| plasma des-acyl ghrelin concentration | pre -prandial | des-acyl ghrelin concentration (fmol/ml) |
| plasma glucose concentration | pre -prandial | glucose concentration (mg%) |
| plasma triglycerides concentration | pre-prandial | triglycerides concentration (mg%) |
| plasma total cholesterol concentration | pre -prandial | total cholesterol concentration (mg%) |
| ghrelin proteolysis | pre -prandial | plasma proteolytic activity of ghrelin (relative units) |
| ghrelin deacylation | pre -prandial | plasma desacylase actvitity of ghrelin (relative units) |
| leptin receptor gene expression in peripheral blood mononuclear cells | pre-prandial | leptin receptor gene expression in peripheral blood mononuclear cells (relative expression) |
| insulin receptor gene expression in peripheral blood mononuclear cells | pre-prandial | insulin receptor gene expression in peripheral blood mononuclear cells (relative expression) |
| ghrelin receptor gene expression in peripheral blood mononuclear cells | pre-prandial | ghrelin receptor gene expression in peripheral blood mononuclear cells (relative expression) |
| hunger sensation | pre-prandial and 15, 30, 45 and 60 min post-prandial | visual analog scale (cm) |
| fullness sensation | pre-prandial and 15, 30, 45 and 60 min post-prandial | visual analog scale (cm) |
| desire to eat sensation | pre-prandial and 15, 30, 45 and 60 min post-prandial | visual analog scale (cm) |
| prospective food consumption sensation | pre-prandial and 15, 30, 45 and 60 min post-prandial | visual analog scale (cm) |
Countries
Argentina
Outcome results
None listed