Osteosarcoma
Conditions
Keywords
ESWT, Cell proliferation, Cell viability
Brief summary
The idea of treating different deformities or diseases in the maxillofacial region with Extracorporeal Shockwave Therapy (ESWT) has recently become popular. It has been widely used in medical practice for the management of urolithiasis, cholelithiasis and in haead and neck region for sialolithiasis. The present study 'Extracorporeal Shock Wave Therapy on Osteoblast-Like Cells In-vitro study' will be done to explore and evaluate the effect of shockwaves. Further, Osteoblast-like cells will be assessed for Cell - Cell interaction and Cell Viability. It will be assessed using a scratch assay, Mtt assay and ATP analysis.
Detailed description
Introduction: Extracorporeal Shockwave Therapy (ESWT) has recently become popular. Shockwave has been widely used in medical practice for the management of urolithiasis, cholelithiasis and in haead and neck region for sialolithiasis. Aim: This study 'Extracorporeal Shock Wave Therapy on Osteoblast-Like Cells In-vitro study' will be done to explore and evaluate the effect of shockwaves. Further, Osteoblast-like cells will be assessed for Cell - Cell interaction and Cell Viability. Methodology: In this in-vitro study, 20 Osteoblast - like cells will be included. 10 samples to be considered for Control group and the remaining 10 samples considered as Test group. Extracorporeal shockwave will be applied to the Test samples. They will be further analysed using wound scratch assay which is used to assess Cell proliferative rate and scratch closure through live imaging. MTT assay and ATP assay used to determine Cell viability after exposure to shockwaves. The cell migration and cell viability after exposure to shockwaves will determine its effects on osteoblast-like cells.
Interventions
Extracorporeal shockwave device generates true shock waves which are characterized by a sudden rise in pressure followed by an exponential decay. This is different from the commercially available shockwave devices in which they use piezoelectric based mechanisms which generate high frequency waves.
DEVICENo ESWT
the osteoblast like cells are not exposed to shockwaves
Sponsors
Krishnadevaraya College of Dental Sciences & Hospital
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
NONE
Intervention model description
test group - application of shockwave on osteoblast control group - No shockwave on osteoblast
Eligibility
Sex/Gender
ALL
Healthy volunteers
Yes
Inclusion criteria
* Cell cultures with a confluency of 70%-80%
Exclusion criteria
* Contaminated cell cultures
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Scratch assay | 72hours | cell migration |
| MTT Assay and ATP analysis | 74hrs | Cell viability |
Countries
India
Outcome results
None listed