Neurodegenerative Diseases
Conditions
Brief summary
The goal of this observational study is to evaluate and to correlate melatonin levels in plasma, saliva and cerebrospinal fluid (CSF) samples of patients undergoing lumbar puncture diagnostic for specific neurological diseases.
Interventions
OTHERblood and saliva sample and lumbar puncture diagnostic for specific neurological diseases
Saliva was collected using Salivettes (Sarsted, Newton, NC, USA) in dim light. CSF was obtained in the morning after overnight fasting by lumbar puncture at the L3/L4 or L4/L5 interspace. Blood samples were drawn in fasting conditions. Plasma was separated by centrifugation.
Sponsors
Istituto Auxologico Italiano
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
50 Years to 85 Years
Healthy volunteers
No
Inclusion criteria
* Neurodegenerative disease
Exclusion criteria
* Age \< 18 years * Pregnancy * Coagulations disorders * Anticoagulant therapy
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Melatonin levels in saliva | Baseline | Analysis of melatonin levels by ELISA kit. Values expressed in pg/ml |
| Melatonin levels in plasma | Baseline | Analysis of melatonin levels by ELISA kit. Values expressed in pg/ml |
| Melatonin levels in cerebrospinal fluid | Baseline | Analysis of melatonin levels by ELISA kit. Values expressed in pg/ml |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Orexin levels in cerebrospinal fluid | Baseline | Analysis of orexin levels by ELISA kit. Values expressed in ng/ml |
| Neurofilaments light chain levels (NFL) in cerebrospinal fluid | Baseline | Analysis of NFL levels by SIMOA kit. Values expressed in pg/ml |
Countries
Italy
Contacts
Primary ContactStefania Cattaldo
Outcome results
None listed