Healthy
Conditions
Brief summary
The purpose of this study was to investigate the effects of vitamin D supplementation on biochemical response and aerobic capacity after strenuous endurance exercise (SEE).
Detailed description
All participants conducted a graded exercise test on an ergometric bicycle to determine the maximal oxygen consumption (VO2max) before and after supplementation, and the speed equivalent to 65% VO2max was conducted for a SEE test by ergometric bicycle for 2 hours, followed by increasing 100 RPM until exhaustion. Venous blood samples were drawn before, immediately after, 2-hour after, 4-hour after, 8-hour after, and 24-hour after the SEE test to determine the concentrations of biomarkers. Data was analyzed by Two-way mixed design ANOVA.
Interventions
DIETARY_SUPPLEMENTVitamin D
Participants received 5,000 IU of vitamin D orally twice daily for 4 weeks
DIETARY_SUPPLEMENTMedium-chain triglycerides
Participants received the same amount of placebo matching vitamin D orally twice daily for 4 weeks
Sponsors
Panion & BF Biotech Inc.
Taipei Medical University
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
DOUBLE (Subject, Investigator)
Masking description
A double-blind, matched-pair study design
Intervention model description
Healthy male participants were divided into vitamin D group and placebo group.
Eligibility
Sex/Gender
MALE
Age
20 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* The volunteers included have conditions as stated below : 1. no history of cardiovascular, liver, kidney and diabetes disease 2. no acute sport injury 3. male adult whose concentration of 25(OH)D is under 30 ng/mL, and maximal oxygen consumption is over 40 ml/kg/min 4. did not participated in any clinical trials or research in the last 3 months before our experiment 5. no supplements were taken during the experimental period 6. were requested to maintain their regular daily life style and avoid alcohol intake
Exclusion criteria
* The volunteers were excluded if they have the situation stated below: 1. history of cardiovascular, liver, kidney and diabetes disease 2. acute sport injury 3. their concentration of 25(OH)D is over 30 ng/mL, or maximal oxygen consumption is under 40 ml/kg/min 4. participated in any clinical trials or experimental research in the last 3 months before our experiment 5. taking supplement during the experimental period 6. did not maintain their regular eating habit or drink alcohol
Design outcomes
Primary
| Measure | Time frame |
|---|---|
| Concentration of Neutrophil | before exercise |
| Concentration ofNeutrophil | immediately after exercise |
| Concentration of eosinophil | before exercise |
| Concentration of basophil | before exercise |
| Concentration of monocyte | before exercise |
| Concentration of lymphocyte | before exercise |
| Concentration of CD4+ | before exercise |
| Concentration of CD8+ | before exercise |
| Concentration of CD19+ | before exercise |
| Concentration of CD56+ | before exercise |
| Concentration of IgA | before exercise |
| Concentration of Cathelicidin | before exercise |
| Concentration of TNF-α | before exercise |
| Concentration of IL-1β | before exercise |
| Concentration of IFN-γ | before exercise |
| Concentration of IL-2 | before exercise |
| Concentration of IL-4 | before exercise |
| Concentration of IL-8 | before exercise |
| Concentration of IL-10 | before exercise |
| Concentration of TBARS | before exercise |
| Concentration of PC | before exercise |
| Concentration of SOD | before exercise |
| Concentration of catalase | before exercise |
| Concentration of GPx | before exercise |
| Concentration of CK | before exercise |
| Concentration of LDH | before exercise |
| Concentration of IL-6 | before exercise |
| Concentration of CRP | before exercise |
Countries
Taiwan
Outcome results
None listed