Brain Injuries, Traumatic
Conditions
Keywords
Traumatic brain injury, Body fluid, Biomarker, Multiomic analysis
Brief summary
The goal of this experimental observation study is to figure out differently expressed biomarkers in body fluid in traumatic brain injury patients. The main questions it aims to answer is: * Which RNA, protein and metabolites are differently expressed in lesion tissues? * What molecular mechanism is participated in TBI? Participants will be treated by emergency operation, and their body fluid samples will be collected in emergency room and during the operation.
Interventions
OTHERType of disease
Different types of pathogen: brain injury by trauma or hydrocephalus due to various diseases.
Sponsors
RenJi Hospital
Study design
Observational model
OTHER
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
16 Years to 65 Years
Healthy volunteers
No
Inclusion criteria
1. Age \>18 and \<65 2. Within 12 hours after brain injury 3. Control group is hydrocephalus 4. GCS ≥3 and ≤8 5. Closed brain injury with frontotemporal contusion 6. Need emergency intracranial hematoma evacuating or cerebrospinal fluid shunting operation 7. Patient's agents are informed and consent the research
Exclusion criteria
1. With TBI, stroke, ICH or intracranial tumor history 2. Death within 24 hours 3. Immunosuppression state 4. Severe organ dysfunction 5. Complicated infective disease 6. Pregnant
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| RNA biomarkers in patients' blood & cerebrospinal fluid assessed by transcriptome bioinformatic analysis | Immediately after collecting blood & cerebrospinal fluid | Patients' blood and cerebrospinal fluid will be collected in emergency room and operation room respectively. Then these samples will be immediately prepared to be detected through transcriptome sequencing. The results of transcriptome sequencing will be further analyzed and compared with control group to filter potential RNA biomarkers in blood and cerebrospinal fluid of severe TBI patients. |
| Protein biomarkers in patients' blood & cerebrospinal fluid assessed by proteomic bioinformatic analysis | Immediately after collecting blood & cerebrospinal fluid | Patients' blood and cerebrospinal fluid will be collected in emergency room and operation room respectively. Then these samples will be immediately prepared to be detected through proteomics mass spectrometry. The results of proteomic will be further analyzed and compared with control group to filter potential protein biomarkers in blood and cerebrospinal fluid of severe TBI patients. |
| Metabolite biomarkers in patients' blood & cerebrospinal fluid assessed by metabolomic bioinformatic analysis | Immediately after collecting blood & cerebrospinal fluid | Patients' blood and cerebrospinal fluid will be collected in emergency room and operation room respectively. Then these samples will be immediately prepared to be detected through metabolomic mass spectrometry. The results of metabolomic will be further analyzed and compared with control group to filter potential metabolite biomarkers in blood and cerebrospinal fluid of severe TBI patients. |
Countries
China
Contacts
Primary ContactZhenghui He
Outcome results
None listed