Brain Injury Traumatic Severe, Intracranial Hemorrhage, Hypertensive
Conditions
Keywords
Traumatic brain injury, Hypertensive intracranial hemorrhage, Multiomic analysis, Biomarker, Molecular mechanism
Brief summary
The goal of this experimental observation study is to figure out differently expressed biomarkers in lesion tissues in traumatic brain injury or hypertension intracranial hemorrhage patients. The main questions it aims to answer is: * Which RNA, protein and metabolites are differently expressed in lesion tissues? * What molecular mechanism is participated in TBI or ICH? Participants will be treated by emergency operation, and their lesion tissues will be collected during the operation.
Interventions
OTHERType of disease
Different types of pathogen: brain injury by trauma or intracranial hemorrhage due to hypertension.
Sponsors
RenJi Hospital
Study design
Observational model
OTHER
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
16 Years to 65 Years
Healthy volunteers
No
Inclusion criteria
1. Age \>18 and \<65 2. Within 12 hours after brain injury 3. GCS ≥3 and ≤8 4. Closed brain injury with frontotemporal contusion or basal ganglia intracranial hemorrhage 5. Need emergency intracranial hematoma evacuating operation 6. Patient's agents are informed and consent the research
Exclusion criteria
1. With TBI, stroke, ICH or intracranial tumor history 2. Death within 24 hours 3. Immunosuppression state 4. Severe organ dysfunction 5. Complicated infective disease 6. Pregnant
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| RNA biomarkers in TBI or ICH patients' lesion tissues assessed by transcriptome bioinformatic analysis | Immediately after collecting tissues | Patients' lesion tissue will be collected during hematoma evacuation. Then these tissues will be immediately prepared to be detected through transcriptome sequencing. The results of transcriptome sequencing will be further analyzed by bioinformatic analysis to reveal different RNA expressions in lesion tissues of severe TBI and ICH patients as the potential biomarkers. |
| Protein biomarkers in TBI or ICH patients' lesion tissues assessed by proteomic bioinformatic analysis | Immediately after collecting tissues | Patients' lesion tissue will be collected during hematoma evacuation. Then these tissues will be immediately prepared to be detected through proteomics mass spectrometry. The results of proteomic will be further analyzed by bioinformatic analysis to reveal different protein expressions in lesion tissues of severe TBI and ICH patients as the potential biomarkers. |
| Metabolite biomarkers in TBI or ICH patients' lesion tissues assessed by metabolomic bioinformatic analysis | Immediately after collecting tissues | Patients' lesion tissue will be collected during hematoma evacuation. Then these tissues will be immediately prepared to be detected through metabolomic mass spectrometry. The results of metabolomic will be further analyzed by bioinformatic analysis to reveal different metabolite expressions in lesion tissues of severe TBI and ICH patients as the potential biomarkers. |
Countries
China
Contacts
Primary ContactZhenghui He, MD
Outcome results
None listed