Glioblastoma, Glioma, Malignant
Conditions
Keywords
glioma stem-like cells, brain organoids, brain invasion, ciliogenesis
Brief summary
In patients operated for glioblastoma, glioma stem-like cell lines will be obtained from tumor tissue, and IPSCs from skin fibroblasts or PBMCs. Brain organoids will be generated from IPSCs and co-cultured with IPSCs to study brain invasion and ciliogenesis. 3D genome architecture of glioma stem-like cells will be investigated. Gene modulation and pharmacologic strategies to inhibit invasion and restore ciliogenesis will be explored.
Interventions
OTHERBiological sample collection
Collection of biological samples
Sponsors
Catholic University of the Sacred Heart
Istituto Superiore di Sanità
Heinrich-Heine University, Duesseldorf
Fondazione Policlinico Universitario Agostino Gemelli IRCCS
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
No
Inclusion criteria
o be enrolled in the study patients must: 1. Have a radiological diagnosis of supratentorial glioblastoma, or 2. Have a radiological diagnosis of first recurrence of a primary supratentorial glioblastoma (for which a formal histopathologic diagnosis of glioblastoma had made at first surgery), according with RANO criteria (Wen, 2010); 3. Be a candidate to neurosurgery for glioblastoma; 4. Be of an age of 18 years or above; 5. Provide written informed consent for participation to the study.
Exclusion criteria
To be enrolled in the study patients must not: 1. Have not enough pathological material removed at surgery available both for mandatory routine histopathological diagnosis and for the present study, as judged by the Principal Investigator; 2. Have not a definitive pathological diagnosis of a primary supratentorial glioblastoma, according with 2016 WHO classification.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Invasion | through study completion, an average of 4 years | Distance travelled by glioma stem cells into brain organoids |
| Gene assessment | through study completion, an average of 4 years | Identification of genes responsible of GSC invasion, among those reportedly involved in self-renewal and ciliogenesis |
Countries
Italy
Contacts
Primary ContactRoberto Pallini, MD, PhD
Backup ContactLucia Ricci-Vitiani, PhD
Outcome results
None listed