Oxidative Stress
Conditions
Keywords
Oxidative stress, Honey, Antioxidants, Health
Brief summary
The aim of the present clinical study is to estimate the efficacy of a honey-based gel, derived from the wider area of Pindos in Greece, in regulating oxidative stress biomarkers and the physiological profile of healthy adults.
Detailed description
Introduction: Honey contains about 200 compounds such as sugars, proteins, vitamins, water, free amino acids, enzymes, minerals, and many phytochemicals. Due to its composition, enriched with many bioactive ingredients, several studies have established its antimicrobial, antiviral, anticancer, antidiabetic and antioxidant properties. This multilayer activity of honey has been proposed for the protection against pathologies interrelated with the cardiovascular, nervous, respiratory, and gastrointestinal system. Purpose: This study aims to investigate the effects of a novel natural honey-based gel consumption on oxidative stress biomarkers, blood chemistry and physiological characteristics in healthy individuals. Methodology: A total of 20 healthy men and women will participate in the study. Participants will give their informed consent after they will be informed about the purposes, procedures, risks and benefits associated with the study. Participants will consume 70 g/day of the honey-based gel, distributed in 2 equal doses (in the morning and evening) for 14 days. At baseline and post-intervention, volunteers will be assessed for their physiological profile and will provide a resting blood sample for the assessment of oxidative stress and blood chemistry biomarkers as well as their physiological profile. Participants will be asked to record their diet for 3 days prior to the study and they will be asked to follow the same dietary pattern for 3 days before the post-intervention assessments.
Interventions
DIETARY_SUPPLEMENTHoney-based gel supplementation
70 g/day of a honey-based gel divided into 2 equal doses of 35 gr (in the morning and evening) for a total duration of 14 days
Sponsors
University of Thessaly
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 65 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy Individuals aged 18-65 years * Non-smokers
Exclusion criteria
* Musculoskeletal injury * Dietary supplements * Medication * Allergy in bee pollen
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in body weight | Change from baseline to 14 days | Body weight will be measured using a digital scale |
| Change in body fat | Change from baseline to 14 days | Body fat will be measured by bioelectrical impedance analysis |
| Change in glutathione concentration of erythrocytes | Change from baseline to 14 days | Glutathione concentration will be analyzed in erythrocytes by spectrophotometry |
| Change in catalase enzyme activity of erythrocytes | Change from baseline to 14 days | Catalase enzyme activity will be analyzed in erythrocytes by spectrophotometry |
| Change in total antioxidant capacity of plasma | Change from baseline to 14 days | Total antioxidant capacity will be analyzed in plasma by spectrophotometry |
| Change in thiobarbituric acid reactive substances of plasma | Change from baseline to 14 days | Thiobarbituric acid reactive substances will be analyzed in plasma by spectrophotometry |
| Change in protein carbonyls of plasma | Change from baseline to 14 days | Protein Carbonyls will be analyzed in plasma by spectrophotometry |
| Change in resting heart rate | Change from baseline to 14 days | Resting heart rate will be measured using a heart rate sensor |
| Change in diastolic and systolic blood pressure | Change from baseline to 14 days | Diastolic and systolic blood pressure will be measured using a manual sphygmomanometer |
| Change in waist and hip circumference | Change from baseline to 14 days | Waist and hip circumference will be assessed using a tape measure |
| Change in complete blood count | Change from baseline to 14 days | White blood cells, lymphocytes, monocytes, granulocytes, red blood cells and platelets will be analyzed in whole blood |
Countries
Greece
Outcome results
None listed