Muscle Injury
Conditions
Brief summary
The study is a 2-week human study where 40 patients who are scheduled to undergo reconstructive knee surgery are randomized to administration of GH or placebo following or without neuromuscular electrical stimulation of hamstring muscles.The overall aim is to determine, the role of muscle connective tissue protein synthesis in muscle injury and repair.
Interventions
PROCEDUREElectrical stimulation
Experimentally induced muscly injury via. neuromuscular electrical stimulation of the hamstring muscles
Daily injection of growth hormone
OTHERControl (No electrical stimulation, nor growth hormone)
No electrical stimulation, nor growth hormone
Sponsors
Bispebjerg Hospital
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
TRIPLE (Subject, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy men and women scheduled to undergo reconstructive knee surgery * BMI 18-35
Exclusion criteria
* Former or current use of growth hormone or anabolic steroids * Use of corticosteroids in the past 3 months * Use of any medication known to affect muscle or tendon turnover * Former participation in a study using deuterated water * Chronic diseases
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Difference in muscle and myotendinous junction protein synthesis in regenerating vs. control muscle | 2 years | Fractional synthesis rates based on D2O (deuterated water) intake and deuterium labelled alanine in tissue |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Single protein synthesis in regenerating vs. control muscle and myotendinous junction tissue | 2 years | Dynamic proteome profiling fractional synthesis rates based on D2O (deuterated water) intake and deuterium labelled alanine in single proteins |
| Single protein synthesis in regenerating muscle and myotendinous junction tissue with or without growth hormone | 2 years | Dynamic proteome profiling fractional synthesis rates based on D2O (deuterated water) intake and deuterium labelled alanine in single proteins |
| Tendon tissue synthesis from injured muscle vs. control muscle | 2 years | FSR based on D2O intake and D-alanine label in tissue |
| Difference in muscle protein synthesis in regenerating muscle, myotendinous junction tissue with or without growth hormone | 2 years | Fractional synthesis rates based on D2O (deuterated water) intake and deuterium labelled alanine in tissue |
| Difference in muscle protein synthesis in regenerating vs. control muscle, myotendinous junction, and tendon tissue | 2 years | Fractional synthesis rates based on D2O (deuterated water) intake and deuterium labelled alanine in tissue |
| Number of satellite cells, fibroblasts and immune cells in renereting vs. control tissue, with and without growth hormone | 2 years | Histochemical staining of muscle cross sections |
| Mechanical properties of tissue with and without growth hormone | 2 years | Ex vivo mechanical stress test |
| Difference in muscle protein synthesis in regenerating muscle, myotendinous junction, and tendon tissue with or without growth hormone | 2 years | Fractional synthesis rates based on D2O (deuterated water) intake and deuterium labelled alanine in tissue |
Countries
Denmark
Contacts
Primary ContactGrith Stougaard Højfeldt, PhD
Outcome results
None listed