Breast Milk Collection, Overweight and Obesity
Conditions
Keywords
Exercise
Brief summary
The investigators will determine the acute effect of exercise on breastmilk composition. Participants will come in to the lab on three different days, out of which they will exercise on two of these days and rest in the lab on the third day. The conditions will be randomly allocated to each participant. Breastmilk samples will be obtained in the morning of each test day, as well as immediately after, one hour after and four hours after the exercise/rest condition. Dietary intake will be standardized on the test days.
Interventions
BEHAVIORALModerate intensity exercise
Treadmill walking/running with moderate intensity
BEHAVIORALHigh intensity exercise
Treadmill walking/running with high intensity
Sponsors
St. Olavs Hospital
Norwegian University of Science and Technology
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Outcomes Assessor)
Masking description
The personnel who will be analysing the milk samples will be blinded to which condition the samples were obtained from and the time-point.
Intervention model description
Sequential assignment
Eligibility
Sex/Gender
FEMALE
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
* 6 weeks postpartum * Exclusively breastfeeding * Term birth * Singleton baby
Exclusion criteria
* Known cardiovascular disease * Known type 1 or type 2 diabetes * Limited ability to exercise
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Breastmilk metabolite composition | Change from before exercise to after exercise (+0 hour, +1 hour, and +4 hours) | Global metabolomics profiling using MS/MS technology |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Adiponectin | Change from before exercise to after exercise (+0 hour, +1 hour, and +4 hours) | Concentration |
| Leptin | Change from before exercise to after exercise (+0 hour, +1 hour, and +4 hours) | Concentration |
| Insulin Growth Factor-1 | Change from before exercise to after exercise (+0 hour, +1 hour, and +4 hours) | Concentration |
| Insulin | Change from before exercise to after exercise (+0 hour, +1 hour, and +4 hours) | Concentration |
| Ghrelin | Change from before exercise to after exercise (+0 hour, +1 hour, and +4 hours) | Concentration |
| Cytokine profiling (Multiplex 27) | Change from before exercise to after exercise (+0 hour, +1 hour, and +4 hours) | Concentration |
| Complex lipids composition | Change from before exercise to after exercise (+0 hour, +1 hour, and +4 hours) | Global metabolomics profiling using MS/MS technology |
Other
| Measure | Time frame | Description |
|---|---|---|
| Fat mass in kg | At baseline | Impedance scale |
| Muscle mass in kg | At baseline | Impedance scale |
| Height in metres | At baseline | Stadiometer |
| Body mass in kg | At baseline | Impedance scale |
| Cardiorespiratory fitness | At baseline | Peak oxygen uptake |
Countries
Norway
Outcome results
None listed