Healthy, Metabolism, Microbiome
Conditions
Keywords
Microbiome,, maternal and offspring, healthy
Brief summary
This study is planned to be recruited in two hospitals in South China, Zhujiang Hospital of Southern Medical University and Zhongshan Boai Hospital Recruit participants, plan to recruit obstetrics and child health departments in study hospitals, meet inclusion and discharge criteria 1\ 3 infants, a total of 360, recruited by Pearl River Hospital of Southern Medical University and Zhongshan Boai Hospital 180 people each.
Detailed description
This is a case-control study. By collecting fecal samples of infants and young children in the observation group and control group at different time points (0\ 1 years old, 1\ 2 years old, 2\ 3 years old), the intestinal flora of infants and young children was detected by 16S rDNA sequencing, and fecal calprotectin (CP) and secretory IgA (SIgA) were detected by ELISA. To study the effects of exposure factors on intestinal flora and immunity in offspring.
Interventions
DRUGantibiotics
Reproductive tract GBS colonization group of mothers during pregnancy，Use of antibiotics
Sponsors
Zhongshan Bo Ai Hospital
Zhujiang Hospital
Study design
Observational model
COHORT
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
37 Weeks to 41 Weeks
Inclusion criteria
* Infants:37-41 weeks of age, born through vaginal delivery, 2500g\<= birth weight \< 4000g,0-3 years of age; * The mother: birth ranging in age from 18 to 40 years old, the single pregnancy, pregnancy 35 -41 weeks to complete the GBS screening; * The guardian agrees to participate in the study voluntarily.
Exclusion criteria
* Infants and children with serious congenital abnormalities, infections or clinical diseases; * Antibiotics should be used in infants within 2 weeks, probiotics, prebiotics and biostime should be supplemented, or long-term drug treatment should be given for digestive, immune, blood and other diseases; * Infant mothers: childbirth after long-term drug treatment due to digestive, immune, blood and chronic diseases during pregnancy; Or infants who were breastfed within 12 hours of antibiotic use after delivery; * Any medical or non-medical condition that the investigator deems inappropriate to participate in.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Calprotectin | Fecal samples of infants and young children in the observation group and control group at different time points (0~1 years old) of offspring were collected | Analyze the effect of intrapartum antibiotics on the prevention of early-onset group B streptococcal diseases on the cailprotectin of offspring. |
| Intestinal flora | Fecal samples of infants and young children in the observation group and control group at different time points (0~1 years old) of offspring were collected | Analyze the effect of intrapartum antibiotics on the prevention of early-onset group B streptococcal diseases on the gut microbiome. |
| Secretory IgA | Fecal samples of infants and young children in the observation group and control group at different time points (0~1 years old) of offspring were collected | Analyze the effect of intrapartum antibiotics on the prevention of early-onset group B streptococcal diseases on the secretory IgA of offspring. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Secretory IgA | Fecal samples of infants and young children in the observation group and control group at different time points 1~2 years of offspring were collected | Analyze the effect of intrapartum antibiotics on the prevention of early-onset group B streptococcal diseases on the secretory IgA of offspring. |
| Intestinal flora | Fecal samples of infants and young children in the observation group and control group at different time points 1~2 years of offspring were collected | Analyze the effect of intrapartum antibiotics on the prevention of early-onset group B streptococcal diseases on the gut microbiome. |
| Calprotectin | Fecal samples of infants and young children in the observation group and control group at different time points 1~2 years of offspring were collected | Analyze the effect of intrapartum antibiotics on the prevention of early-onset group B streptococcal diseases on the cailprotectin of offspring. |
Other
| Measure | Time frame | Description |
|---|---|---|
| Intestinal flora | Fecal samples of infants and young children in the observation group and control group at different time points 2~3 years old of offspring were collected | Analyze the effect of intrapartum antibiotics on the prevention of early-onset group B streptococcal diseases on the gut microbiome. |
| Calprotectin | Fecal samples of infants and young children in the observation group and control group at different time points 2~3 years old of offspring were collected | Analyze the effect of intrapartum antibiotics on the prevention of early-onset group B streptococcal diseases on the secretory IgA of offspring. |
| Secretory IgA | Fecal samples of infants and young children in the observation group and control group at different time points 2~3 years old of offspring were collected | Analyze the effect of intrapartum antibiotics on the prevention of early-onset group B streptococcal diseases on the secretory IgA of offspring. |
Countries
China
Outcome results
None listed