n-3 Polyunsaturated Fatty Acids
Conditions
Keywords
n-3 polyunsaturated fatty acids, vascular function, endothelium, oxidative stress, inflammation, microcircularion, macrocirculation
Brief summary
Evidence from multiple experimental, epidemiological, and clinical studies indicate that n-3 polyunsaturated fatty acids (n-3 PUFAs) may exert their cardiovascular (CV) protective effect, at least in part, by improving vascular endothelium-dependent function. Previously, the investigators demonstrated the significant beneficiary effect of consumption of n-3 PUFA-enriched hen eggs on microvascular endothelium-dependent reactivity, even in young healthy individuals. The hypothesis of this research is that the consumption of n-3 PUFAs in the form of functionally enriched chicken meet has a beneficial effect on vascular reactivity measured in different vascular beds, and the lipid profile with a positive effect on the reduction of oxidative stress and inflammatory response in healthy sedentary individuals. The main goal of this study is to investigate the influence of n-3 PUFAs functionally enriched chicken meet on vascular and endothelial function in a population of healthy young subjects and active athletes.
Interventions
DIETARY_SUPPLEMENTN-3 PUFAs Chicken Meet
Intake of n-3 PUFAs enriched chicken meet for three weeks.
DIETARY_SUPPLEMENTRegular Chicken Meet
Intake of regular chicken meet for three weeks.
Sponsors
Josip Juraj Strossmayer University of Osijek
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
PREVENTION
Masking
DOUBLE (Subject, Investigator)
Eligibility
Sex/Gender
ALL
Age
18 Years to 39 Years
Healthy volunteers
Yes
Inclusion criteria
* young healthy sedentary individuals - did not perform regular physical activities for at least 12 months before conducting the study * young healthy active athletes - have a minimum of 5 training sessions per week (mainly endurance training) for at least 12 months prior to conducting the study
Exclusion criteria
* smoking * hypertension * coronary disease * diabetes * hyperlipidemia * kidney damage * cerebrovascular and peripheral artery diseases * and any other disease that could have an impact on vascular and endothelial function
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Microvascular endothelial function | 3 weeks | Cutaneous microvascular blood flow will be measured by Laser Doppler Flowmetry in response to vascular occlusion (post occlusive reactive hyperaemia - PORH), in response to iontophoresis of acetylcholine (ACh) and local thermal hyperemia (LTH). |
| Macrovascular endothelial function | 3 weeks | Measurement of flow mediated dilation (FMD) of brachial artery using ultrasound. |
| Cerebrovascular reactivity | 3 weeks | Measurement of cerebrovascular blood flow using transcranial color Doppler (TCD). |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Serum Pro- and Anti-Inflammatory Cytokines, Chemokines, Growth Factors and Soluble Cell Adhesion Molecules Protein Concentration | 3 weeks | Measurement of serum protein concentration (pg/mL) of various pro- and anti-inflammatory cytokines, chemokines, growth factors and soluble cell adhesion molecules by kits and panels for multiplex protein quantitation using the Luminex 200 instrument platform. |
| Lipid profile | 3 weeks | Measurement of plasma cholesterol, HDL cholesterol, LDL cholesterol and triglycerides level. |
| Body fluid status | 3 weeks | Body fluid status measurement by a four-terminal portable impedance analyzer. Based on the value of skin resistance (Ohm) empirically derived formulas (the original manufacturer's software) will be used to calculate the estimated Total Body Water (TBW L), Extracellular Water (ECW L), Intracellular Water (ICW L), Plasma Fluid (PF L) and Interstitial Fluid (IF L). |
| Body composition | 3 weeks | Body composition and body fluid status measurement by a four-terminal portable impedance analyzer. Based on the value of skin resistance (Ohm) empirically derived formulas (the original manufacturer's software) will be used to calculate the estimated Fat Free Mass (FFM kg) and Fat (Fat Mass kg). |
| Oxidative stress status | 3 weeks | Measurement of Thiobarbituric Acid Reactive Substances (TBARS). |
| Systemic hemodynamics | 3 weeks | Non-invasive assessment of systemic hemodynamics using impedance cardiography (CardioScreen 2000) - SV Stroke Volume (mL), CO Cardiac Output (L/min) and SVRI Systemic Vascular Resistance Index (dyn.s.cm-5 m2). |
Countries
Croatia
Contacts
Primary ContactInes Drenjancevic
Outcome results
None listed