Microbial Colonization, Neonatal Infection, Viral Infection
Conditions
Keywords
Metatranscriptomics, Microbiome, Newborn
Brief summary
The goal of this interventional study is to learn about nasal microbiome and viral infections in newborns hospitalised in neonatology. The main questions it aims to answer are : * how often are newborns infected by viruses in neonatology ? * how does the microbiome develop during first weeks of life ? * how does microbiome and viruses interacts together ? * is there a link between viral infection, microbiome, and medical complications during hospitalisation in neonatology ? Participants will have a nasal swab taken each week during their hospitalisation in neonatology, and researchers will take medical data from the medical chart.
Interventions
DIAGNOSTIC_TESTNasal swab
We will take nasal swabs from anterior and middle nostril, once a week for each newborn included.
Sponsors
University Hospital, Caen
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
No minimum to 48 Hours
Healthy volunteers
No
Inclusion criteria
* newborn hospitalised in neonatal intensive care unit during the inclusion period * parents have been informed about the study (speaking french) and have signed the informed consent. * affiliated to the social security system.
Exclusion criteria
* parental refusal * minor parents or under judicial protection * newborn who already have been discharded home before inclusion * hospitalisation after the first 48 hours of life * only palliative care
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in microbiome diversity | Day 0, day 7, day 14, and each week until discharge (maximum duration : 3 months for each newborn). | Shannon diversity index |
| Change in microbiome abundance | Day 0, day 7, day 14, and each week until discharge (maximum duration : 3 months for each newborn). | Number of reads for a given taxonomy. |
| Virus presence | Day 0, day 7, day 14, and each week until discharge (maximum duration : 3 months for each newborn). | Molecular detection of respiratory viruses using multiplex PCR. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Occurence of bronchopulmonary dysplasia | At discharge from neonatology. | assessed by clinical recording on discharge from neonatology |
| Change in expression of local innate immunity markers | Day 0, day 7, day 14, and each week until discharge (maximum duration : 3 months for each newborn). | Mesure of the expression of the interferon-stimulated genes (ISGs), anti-viral genes, and the inflammasome pathway. |
Countries
France
Outcome results
None listed