Salivary Cystatin S Levels in a Group of Egyptian Children

The Evidence of Early Childhood Caries Prediction by Salivary Cystatin S Levels in a Group of Egyptian Children: a Case Control Study

Status

Active, not recruiting

Phases

Unknown

Study type

Observational

Source

ClinicalTrials.gov

Registry ID

NCT05716867

Enrollment

Registered

2023-02-08

Start date

2023-12-01

Completion date

2025-05-10

Last updated

2025-02-17

For informational purposes only — not medical advice. Sourced from public registries and may not reflect the latest updates. Terms

Conditions

Early Childhood Caries

Keywords

early childhood caries, salivary cystatin S protein, salivary buffer capacity

Brief summary

Aim of the study 1. Measuring the levels of salivary cystatin S protein and salivary buffering capacity to determine their relation to early childhood caries. 2. Predection of early childhood caries by using salivary cystatin S levels with demographic, clinical and nutrition habits characteristics of the participants.

Interventions

BIOLOGICALMeasurement of salivary cystatin S protein levels in saliva

The human cystatin S enzyme-linked immunosorbent assay (ELISA) kit will be used to measure the amounts of cystatin S in saliva for cases and controls

Study design

Observational model

CASE_CONTROL

Time perspective

RETROSPECTIVE

Eligibility

Sex/Gender

ALL

Age

36 Months to 71 Months

Healthy volunteers

Yes

Inclusion criteria

* Medically free children aged 36 to 71 months with one or more decayed (noncavitated or cavitated lesions), missing, or filled teeth due to caries in any primary tooth(cases). * Medically free children aged 36 to 71 months with no decayed, missing, or filled teeth due to caries in any primary tooth(controls).

Exclusion criteria

* Children receiving antibiotic therapy within three month and fluoride prophylaxis within one year. * Parents who will refuse to participate or refuse to sign the informed consent. * Children who will not agree or cooperate with participation.

Design outcomes

Primary

Measure	Time frame	Description
Levels of salivary cystatin S protein	2 months	Levels of salivary cystatin S protein measured by Human cystatin S enzyme linked immunosorbent assay (ELISA) kit (unit ng/ml)

Secondary

Measure	Time frame	Description
Buffer Capacity	2 months	Buffer Capacity measured by PH meter
Demographic, clinical and nutrition habits characteristics of participants Interview	2 months	Demographic, clinical and nutrition habits characteristics of participants, using interview

Countries

Egypt

Outcome results

None listed

Source: ClinicalTrials.gov · Data processed: Feb 4, 2026