Blood Glucose Fluctuations, Hunger
Conditions
Brief summary
The aim of this cross-over intervention study is to investigate the influence of the sweet-modulating substance hesperetin in combination with sucrose in comparison to an equally sweet-tasting sucrose-only solution on markers of energy metabolism.
Detailed description
The study is designed as a randomised cross-over intervention study with two arms. The aim is to investigate the influence of a 10% sucrose solution, corresponding to the sugar content of a conventional soft drink, compared to an equally sweet-tasting 7% sucrose solution in combination with 50 mg/L of the polyphenol hesperetin, on time-dependent blood glucose fluctuations and ad libitum energy intake in metabolically healthy male volunteers. To investigate the underlying mechanisms, the individual appetite score, metabolic and hormonal responses to the interventions and circulating extracellular vesicles and their miRNA composition will be measured. In addition, the participants' individual thresholds for sweet taste, preference and consumption for sweet-tasting foods such as sugar and sweeteners, and their body composition will be recorded as potential influencing factors.
Interventions
OTHERSucrose
10% sucrose corresponding to a regular soft drink
DRUGHesperetin
increases sweetness of 7% sucrose solution to match sweetness of 10% sucrose solution
Sponsors
University of Vienna
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
DOUBLE (Subject, Outcomes Assessor)
Eligibility
Sex/Gender
MALE
Age
18 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
* male * healthy * non-smoking * normal taste responses towards sweet taste
Exclusion criteria
* female * regular smokers * disturbed glucose and/or lipid metabolism * regular intake of medication * known allergies against one of the test compounds * ageusia * alcohol or drug addiction * intake of antibiotics within the past 2 months
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in blood glucose concentration | 15, 30, 60, 90, and 120 minutes after drinking the test solution | Change compared to baseline in blood glucose concentration \[mg/dL\] in plasma samples after drinking the test solution |
| Change in appetite score | Difference between before and 120 minutes after drinking of the test solution | Change compared to baseline in the subjective rating of the individual feeling of hunger, fullness, as well as appetite for a snack or for something sweet on a digital visual analog scale in comparison to baseline |
| Change in food intake | 120 minutes after drinking of the test solution | Ad libitum food intake from a standardized breakfast |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Change in satiety hormones | 15, 30, 60, 90, and 120 minutes after drinking the test solution | Change compared to baseline in Polypeptide Y, grehlin, cholecystokinin, and serotonin concentrations \[mmol/L\] in plasma samples after drinking the test |
| Change in blood glucose regulating hormones | 15, 30, 60, 90, and 120 minutes after drinking the test solution | Change compared to baseline in Insulin, Glucagon-like peptide 1, and Gastric inhibitory polypeptide concentrations \[mmol/L\] in plasma samples after drinking the test solution |
| Change in circulating miRNA | 15, 30, 60, 90, and 120 minutes after drinking the test solution | Change compared to baseline in circulating extracellular vesicles and their miRNA composition |
Countries
Austria
Outcome results
None listed