Skeletal Muscle Physiology
Conditions
Brief summary
The purpose of the project is investigate muscle memory and underlying mechanisms in humans following resistance training and use of the anabolic substance clenbuterol.
Interventions
DRUGClenbuterol
Participants ingest 80 micrograms clenbuterol daily during the initial 8-week period. This period is followed by a washout of 16 weeks with no drug and resistance training. Thereafter, an 8-week period with supervised resistance training is commenced, with no drug.
DRUGPlacebo
Participants ingest placebo tablets daily during the initial 8-week period. This period is followed by a washout of 16 weeks with no drug and resistance training. Thereafter, an 8-week period with supervised resistance training is commenced, with no drug.
Sponsors
Morten Hostrup, PhD
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
QUADRUPLE (Subject, Caregiver, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
18 Years to 40 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy men and women * BMI of \<26 and normal ECG and blood pressure
Exclusion criteria
* Smoking * Chronic disease, * Use of prescription medication * Pain due to current or previous musculoskeletal injury * Resistance training more than once per week in the 12 months leading up to the intervention * Current or previous use of prohibited anabolic substances
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Fiber cross-sectional area | The change in fiber cross-sectional is assessed at baseline, 8, 24 and 32 weeks | Immunohistochemistry to determine fiber cross-sectional area |
| Myonuclei | The change in myonuclei is assessed at baseline, 8, 24 and 32 weeks | Immunohistochemistry to determine number of myonuclei |
| Skeletal muscle proteome | The change in the muscle proteome is assessed at baseline, 8, 24 and 32 weeks | Pathway enrichment analysis of the muscle proteome using mass-spectrometry based proteomics |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Sprint performance | The change in sprint performance is assessed at baseline, 8, 24 and 32 weeks | Work performed (W) during a sprint on a bicycle ergometer |
| Lean mass | The change in lean mass is assessed at baseline, 8, 24 and 32 weeks | Lean mass assessed by dual X-ray absorptiometry (DXA) |
| Satellite cells | The change in satellite cells is assessed at baseline, 8, 24 and 32 weeks | Immunohistochemistry to determine number of satellite cells |
| Isometric muscle strength | The change in maximal voluntary isometric contraction is assessed at baseline, 8, 24 and 32 weeks | Maximal voluntary isometric contraction of m. quadriceps |
| Dynamic muscle strength | The change in 1-repetition maximum is assessed at baseline, 8, 24 and 32 weeks | 1-repetition maximum |
Countries
Denmark
Contacts
Primary ContactMorten Hostrup
Outcome results
None listed