Cancer
Conditions
Keywords
Colorectal cancer, Liver cancer, Squamous cell carcinoma of the head and neck, Esophageal carcinoma, Pancreatic cancer, Ovarian cancer, Bladder cancer, Cervical cancer, Lung cancer, Stomach cancer
Brief summary
We intend to establish an efficient method for plasma cfDNA extraction and Bisulfite transformation to facilitate the detection of DNA methylation status using multiplex fluorescence PCR. Meanwhile, we expect to identify several plasma methylation markers that can be highly sensitive for multi-cancer detection. Finally, we will provide a pan-cancer blood test that is easy to operate, low cost, accurate and easy to promote.
Interventions
DIAGNOSTIC_TESTDNA methylation test
Cell-free DNA were extracted from plasma collected from individuals of cancer and non-cancerous disease groups, then the DNA was bisulfite converted and tested by DNA methylation assay based on real-time PCR platform.
Sponsors
Wuhan Ammunition Life-tech Co., Ltd
Study design
Observational model
CASE_CONTROL
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
18 Years to 80 Years
Healthy volunteers
Yes
Inclusion criteria
\- (1)age \>= 18 years. (2) with high suspicion of cancer diagnosed by endoscopy, other imaging examinations, pathological examinations, etc. (3) no treatment with radiotherapy or chemotherapy.
Exclusion criteria
* (1) received antineoplastic treatment such as radiation/chemotherapy. (2) not preserved as required. (3) contaminated or volume is insufficient. (4) unclear pathological results (5) incomplete patient information.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Sensitivity | immediately after the procedure | The reference standard is the results of histopathological tests |
| Specificity | immediately after the procedure | — |
Countries
China
Contacts
Primary ContactTingting Li, PhD
Outcome results
None listed