Cataract, Pathogenesis
Conditions
Brief summary
To study the molecular pathogenesis of cataract and explore possible treatments，anterior lens capsules were collected from cataract patients
Detailed description
Anterior lens capsules were collected from the patients with DC and ARC, and transparent crystals of cadaveric eyes. M6A epitranscriptomic microarray was performed to investigate the altered m6A modifications and determine the differentially expressed mRNAs. The potential role of dysregulated m6A modification levels was predicted through Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway enrichment analyses. Real-time polymerase chain reaction was performed to identify the dysregulated expression of RNA methyltransferases, demethylases, and readers.
Interventions
None listed
Sponsors
National Natural Science Foundation of China
Eye & ENT Hospital of Fudan University
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 90 Years
Healthy volunteers
No
Inclusion criteria
* diabetic cataract * underwent cataract surgery
Exclusion criteria
* Combined with other eye diseases * underwent other eye surgery
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| m6A modification of mRNAs | 1 month | Epitranscriptomic microarray analysis reveals the differential m6A modification of mRNAs in DC samples |
| expression level of mRNAs | 1 month | the m6A microarray analyses provide data for mRNA expression levels |
| levels of methylated mRNA | 1 month | in vitro validation of the diverse expression levels of methylated mRNA |
| levels of RNA methyltransferase | 1 month | in vitro validation of the diverse expression levels of RNA methyltransferase |
Countries
China
Contacts
Primary ContactLei Cai, MD
Outcome results
None listed