Bladder Cancer
Conditions
Keywords
volatile organic compounds, urine, bladder cancer, GS-MS
Brief summary
The goal of this study to investigate the volatile organic compounds (VOCs) in the urine samples of both healthy individual and bladder cancer patients, using gas chromatography - mass spectrometry. Researchers aim to find a set of certain VOCs specific to bladder cancer and use this set to construct a diagnostic model that can help diagnosing bladder cancer. Participants will be asked to collect their early morning midstream urine in a predesignated container. Researchers will compare urine VOCs from bladder cancer patients and healthy individuals to see if there is any VOC whose concentration differ significantly among the two groups.
Detailed description
Specifically, participants will be asked to collect 35-45 ml of their early morning midstream urine sample in a predesignated polytetrafluoroethylene bottle. Researcher will collect participants' health information, including their: 1)smoking history; 2)comorbidity; 3) history of past urological procedure; 4)medication; 5)pathology diagnosis after surgery, if participants have bladder cancer.
Interventions
DIAGNOSTIC_TESTanalysis of urine volatile organic compounds
gas chromatography - mass spectrometry will be used to assess the composition of VOCs in urine samples of all participants.
Sponsors
First Affiliated Hospital Xi'an Jiaotong University
Study design
Observational model
COHORT
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
18 Years to 80 Years
Healthy volunteers
Yes
Inclusion criteria
* bladder tumor confirmed by cystoscopy and radiology * women of childbearing age with negative urine pregnancy test result * willing to participate in this trial and sign the informed consent form
Exclusion criteria
* patient with 1) uncontrollable urinary tract infection; 2) severe systemic disease; 3) history of malignancy within the past 5 years; 4) pregnancy or lactation * patient involved in other clinical trials * other situations researcher considered to be ineligible for this trial
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Urine volatile organic compounds composition of all urine samples | immediately after GC-MS analysis has been finished | Data derived from mass spectrums of GC-MS measurement of VOC components in urine samples, including VOC components identified comparing obtained data to those in public GC-MS database and the absolute concentration of VOCs identified. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Pathological diagnosis of bladder tumor patients enrolled | Immediately after the release of pathological examination reports by pathology department in the First affiliated hospital of Xi'an jiaotong university | Staging of bladder cancer using American Joint Committee on Cancer (AJCC) TNM system and histology of tumor tissue will be collected, if patient has received surgery during hospitalization. Concerning bladder tumor patients only. |
Countries
China
Outcome results
None listed