Dietary Exposure, Safety Issues
Conditions
Keywords
lumisterol, tachysterol, hydroxy-photoisomers, vitamin D
Brief summary
The European Food Safety Authority has approved many applications for UVB light treated foods (e.g. UVB-exposed button mushrooms) in the last years. The UVB light treatment is used to increase the vitamin D content in foods and improve the vitamin D status of subjects. However, UVB irradiation is accompanied by the formation of vitamin D photoisomers such as lumisterol and tachysterol. The current study aims to investigated whether these vitamin D photoisomers can enter the circulation and are metabolised in humans that consume UVB-treated mushrooms.
Detailed description
according to protocol
Interventions
DIETARY_SUPPLEMENTUVB-exposed button mushrooms
Healthy subjects will be randomized into two groups and received either UVB-exposed button mushrooms or non-UVB-exposed button mushrooms for 7 days. Blood samples from each subject will be taken at baseline (before the intake of the mushrooms, 3 h and 6 h thereafter, and at day 8. Three months later, another blood sample was taken.
Sponsors
Martin-Luther-Universität Halle-Wittenberg
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
TREATMENT
Masking
QUADRUPLE (Subject, Caregiver, Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
18 Years to 65 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy subjects * Age between 18 and 65 years * Body Mass Index in the range of 18.5 to 29.9 kg/m2
Exclusion criteria
* Acute or chronic illnesses (high blood pressure, heart disease, diabetes, kidney disease, liver disease, alcohol dependence, etc.) * Taking medication (except oral contraceptives) * Pregnancy or breastfeeding * Food intolerances or allergies to mushrooms and dairy products * Smokers * Visits to solariums or previous holidays in southern countries or in the Alps or other high mountains * Participation in another study * Blood donation during the last 2 months before the start of the study * Dieting * Severe weight loss or weight loss (≥ 3 kg) within the last month
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Circulating vitamin D photoisomers | at baseline (before the intervention), 3 hours postprandial, 6 hours postprandial, at day 8, changes from baseline at 3 months | Plasma concentrations of vitamin D photoisomers (such as lumisterol, tachysterol and their hydroxy derivates) after the consumption of the UVB-exposed versus non UVB-exposed button mushrooms |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Parameters of vitamin D metabolism | at baseline (before the intervention), 3 hours postprandial, 6 hours postprandial, at day 8, changes from baseline at 3 months | Plasma concentrations of vitamin D metabolites (vitamin D2, vitamin D3, 25-hydroxyvitamin D2, 25-hydroxyvitamin D3, 24,25-dihydroxyvitamin D2, calcitriol, ergosterol) |
| Parameters of mineral metabolism | at baseline (before the intervention), 3 hours postprandial, 6 hours postprandial, at day 8, changes from baseline at 3 months | Plasma calcium, phosphate, parathyroid hormone, fibroblast growth factor-23 |
| Plasma lipids | at baseline (before the intervention), 3 hours postprandial, 6 hours postprandial, at day 8, changes from baseline at 3 months | Plasma triglycerides, cholesterol |
| Inflammation markers | at baseline (before the intervention), 3 hours postprandial, 6 hours postprandial, at day 8, changes from baseline at 3 months | Plasma CRP, Interleukin-6 |
| mRNA and protein expression in peripheral mononuclear blood cells | at baseline (before the intervention), 3 hours postprandial, 6 hours postprandial, at day 8, changes from baseline at 3 months | Vitamin D receptor target genes, CYP enzymes |
Contacts
Primary ContactGabriele I Stangl, Prof. Dr.
Outcome results
None listed