Giant Cell Arteritis, Tomography, Optical Coherence
Conditions
Keywords
Giant Cell Arteritis, Temporal Artery Biopsy, Full-field Optical Coherence Tomography
Brief summary
Giant cell arteritis (GCA) is a type of large vessel granulomatous vasculitis responsible for the inflammation of the aorta and the branches of the external carotid, notably temporal arteries. The diagnosis of GCA relies upon the identification of vasculitis following histopathological analysis of temporal artery biopsy (TAB) showing mononuclear cells infiltration, fragmentation of the internal elastic lamina as well as significant intimal hyperplasia. Apart from its lack of sensitivity, one of the weaknesses of TAB is the delay in obtaining the result due to the time required to prepare the sample for histological analysis. Pursuing the idea to improve TAB performances, our group recently demonstrated the use of full-field optical coherence tomography (FF-OCT) to visualize structural changes associated with the inflammatory processes of GCA. The present work suggests a further use of dynamic FF-OCT on TAB for a direct visualization of the mononuclear cells infiltration to ensure rapid on-site diagnosis of GCA.
Interventions
OTHERDynamic full-field optical coherence tomography analysis of temporal artery biopsy
Dynamic full-field optical coherence tomography analysis of temporal artery biopsy in the dermatology department before conventional histopathological analysis
Sponsors
Centre Hospitalier de Mâcon
Centre Hospitalier William Morey - Chalon sur Saône
Study design
Observational model
OTHER
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
50 Years to No maximum
Healthy volunteers
No
Inclusion criteria
* patient \> 50 years of age with suspected giant cell arteritis who received temporal artery biopsy between start study date and primary completion date
Exclusion criteria
* inability to perform dynamic full-field optical coherence tomography observation at the moment of temporal artery biopsy
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Histopathological analysis of healthy temporal artery biopsy with dynamic full-field optical coherence tomography | Outcome measure is assessed 15 days following temporal artery biopsy | Provide a better understanding of the ability of dynamic full-field optical coherence tomography to identify the normal structures of a temporal artery biopsy, i.e. the tripartite architecture with a clear distinction between intima (endothelial cells), media (vascular smooth muscle cells) and adventitia, both internal and external elastic lamina, and vasa vasorum |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Histopathological features of giant cell arteritis with dynamic full-field optical coherence tomography | Outcome measure is assessed 15 days following temporal artery biopsy | Provide a better understanding of the ability of dynamic full-field optical coherence tomography to identify histopathological features of giant cell arteritis, i.e. infiltration of mononuclear cells in the three layers of the artery, fragmentation of the internal elastic lamina, intimal hyperplasia and neoangiogenesis |
Countries
France
Outcome results
None listed