Corneal Neovascularization, Neutrophils
Conditions
Brief summary
The purpose of this study is to determine whether a novel subset of neutrophils is correlated with corneal neovascularization in the patients suffered from ocular chemical injury.
Detailed description
Cornea is an avascular transparent tissue providing refractive power for suitable optical performance. But under the condition of injury or infection, the stable microenvironment of ocular surface has been destroyed. Vessels sprout from the limbus to form corneal neovascularization, becoming an important pathological feature and leading cause of blindness. However, the underlying mechanism particular the inflammation-mediated mechanism remains unclear. With the advancement of single-cell RNA sequencing (scRNA-seq), the investigation of immune cell transcriptomes has obtained great process . Using this advanced method, we identified different immune cell types in the ocular surface microenvironment in corneal neovascularization mouse model. Particularly, we found a specific subset of neutrophils played important role during the corneal angiogenesis. Using the loss-of-function and gain-of-function experiment, we clarified that this novel subset of neutrophils is the major factor that promoting corneal angiogenesis in mouse model. To further explore the relationship between this subset of neutrophils and angiogenesis in human, we plan to detect whether the neutrophil subset is increased in the circulation of chemical burned corneal neovascularization patients. The main purpose of this study is to assess whether the novel subset of neutrophils has correlation with chemical burned corneal neovascularization. Approximately 33 patients and 33 age matched healthy controls will be recruited. The clinical characteristics, 4ml peripheral blood and the images of slit lamp photography will be obtained.
Interventions
None listed
Sponsors
Zhongshan Ophthalmic Center, Sun Yat-sen University
Study design
Observational model
CASE_CONTROL
Time perspective
CROSS_SECTIONAL
Eligibility
Sex/Gender
ALL
Age
18 Years to 65 Years
Healthy volunteers
Yes
Inclusion criteria
Diagnosed ocular chemical injury with or without corneal neovascularization.
Exclusion criteria
1. Those who are diagnosed ocular diseases except for chemical ocular injury, such as uveitis, retinopathy. 2. Those who are diagnosed allergic disease, autoimmune diseases or hematological diseases, infectious disease, such as eczema, systemic lupus erythematosus (SLE), agranulocytosis and viral hepatitis. 3. Those who took immunosuppressive treatments in the past 3 months. 4. Pregnant or nursing women. 5. Those who have serious heart, lung, liver or kidney diseases. 6. Those who have serious systemic diseases. 7. Those who performed ocular surgery (including cataract surgery) in recent three months. 8. Those who have been enrolled in other interventional clinical studies at the same time. 9. Those who are unable to complete the study according to the investigators' requirements.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Changes of angiogenic neutrophils ratio | Inclusion (Day1) and 1 week | Peripheral blood is collected in EDTA coated tubes and the red blood cells are lysed. The cells are firstly blocked and then stained the neutrophil subset markers. Flow cytometric analysis is performed at BD LSRFortessa™ Cell Analyzer and cells are analyzed by FlowJo v10 software. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Corneal Neovascularization Score | Inclusion (Day1) and 1 week | The neovascularization is scored on a scale of 0-3 as previously reported. 0 = no vessels; 1 = neovessels at the corneal limbus; 2 = neovessels spanning approaching the corneal center; 3 = neovessels spanning the central cornea. |
Countries
China
Contacts
Primary ContactZiqi Yang
Outcome results
None listed