Anorexia Nervosa, Anxious Depression, Dysbiosis
Conditions
Keywords
Anorexia nervosa, Dysbiosis, Anxious depression, Intestinal microbiota, Body mass index
Brief summary
The purpose of this study will be to study the association between the level of psychic symptomatic of anorexia nervosa (AN) (intensity of food restriction, symptoms of anxiety and depression) and alteration of host environment symbiosis and the mechanism (dysbiosis of intestinal microbiota, increase of intestinal permeability, immunity alteration and low-grade inflammation).
Detailed description
This is a monocentric study aims to characterise the intestinal microbiota of anorexia nervosa patients with malnutrition, in comparison with the control subject, by DNA sequencing and metagenomic method. The interaction of intetinal microbiota with the host will be studied through the mecanistic studies and various parameters of alteration of symbiosis (intestinal permeability, inflammation) and their association with psychic symptoms of anorexia nervosa. The objective of this approche is to have not only a descrition of genomic of material of sampling, but also an overview of its potential functioning.
Interventions
BIOLOGICALStool and blood samples
Stool and blood samples
Sponsors
Fondation de France
Assistance Publique - Hôpitaux de Paris
Study design
Observational model
CASE_CONTROL
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to No maximum
Healthy volunteers
Yes
Inclusion criteria
For patients: * Patients aged ≥18 years; * Anorexia according to DSM-5 and CIDI (Composite International Diagnostic Interview); * Body mass index (BMI) (P/T2) \< 15; * Hospitalization for nutrition rehabilitation; * Covered by a health insurance; * Informed consent form signed. For Healthy Volunteers: * Aged ≥18 years; * 18.5 \< BMI (P/T2) \< 25; * Covered by a health insurance; * Informed consent form signed.
Exclusion criteria
For patients: * Patients no-responding all criteria of DSM-5 or CIDI scores; * Taken of antibiotic treatment 2 months / or laxativ 3 weeks before hospitalization; * Somatic comorbidity should perturb intestinal microbiota (Crohn's disease, diabetes and all other chronic inflammatory diseases); * Patients under guardianship; * Patients covered by french AME scheme. For Healthy Volunteers: * Any disease should perturb intestinal microbiota; * Recent ponderal variation; * Taken of antibiotic treatment 2 months or laxativ 3 weeks before hospitalization; * Under guardianship; * Covered by french AME scheme.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Eating Disorder | at baseline | By Eating Disorder Inventory (EDI-3) |
| Anxio-depressive symptomatologic assessements by HAD score | at baseline | By HAD score |
| Anxio-depressive symptomatologic assessements by scale of Beck BDI 13 | at baseline | By scale of Beck BDI 13 |
| Anxio-depressive symptomatologic assessements by LSAS Lieboweitz social anxiety scale | at baseline | By LSAS Lieboweitz social anxiety scale |
| Anxio-depressive symptomatologic assessements by MOCI | at baseline | By Maudsley Obsessions and compulsions inventory (MOCI). |
| Anxio-depressive symptomatologic assessements | at baseline | By HAD score, scale of Beck BDI 13, LSAS Lieboweitz social anxiety scale, Maudsley Obsessions and compulsions inventory (MOCI). |
| Physical exercise | at baseline | By Global physical activity questionnaire (GPAQ) |
| Biological parameter | at baseline | By serum serotonin level |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Gene and species richness | at baseline | By 16S rRNA gene sequencing method and Metagenomics |
| Circulating mitochondrial DNA copy number | at baseline | By telemore size and HLA genotyping |
| Heart damage | at baseline | By Left Ventricle Ejection Fraction \< 50% structural parameters on ultrasound |
| Presence of Autistic symptoms (1) | at baseline | By Glasgow Sensory Questionnaire GSQ) |
| Diversity indice | at baseline | By index de Sympson et Shannon |
| EDI | at baseline | QUESTIONNAIRE EDI or EATING DISORDER INVENTORY (Eating disorder inventory 2nd edition (EDI-2) |
| Eating Disorder Diagnostic | at baseline | By Eating Disorder Diagnostic Scale (EDDS)-DSM-5 VERSION (2015 Wiley Periodicals) |
| Eating behaviors assessment | at baseline | By the Detail and Flexibility Questionnaire (DFlex). |
| Presence of Autistic symptoms (2) | at baseline | By The SWedish Eating Assessment for Autism spectrum disorders GSQ) |
| Intestinal permeability | at baseline | By serum zonulin level |
| Immunity alteration | at baseline | By interleukins and CRP level |
| Liver function | at baseline | Cytolyse analysis in serum |
| BMI | at baseline | Calculate of BMI. |
| Body composition | at baseline | By Dexa-Scan. |
| Energy expenditure | at baseline | Calculate of indirect calorimetry and blood parameters |
| Intestinal functional disorders | at baseline | By Francis scale, ,transit with pellets and abdomen x-ray |
Countries
France
Contacts
Primary ContactMouna HANACHI GUIDOUM, MD
Backup ContactJoël DORE
Outcome results
None listed