Connective Tissue, Exercise, Exosomes
Conditions
Brief summary
The proposed study aims to shed light on the function/importance/relevance of exercise-induced changes in exosomes to connective tissues. Exosomes are known to increase robustly in response to exercise. We have previously shown that serum isolated from subjects after they lift heavy weights increases human engineered ligament collagen content and mechanics more than serum from before they lift weight. Further, we showed that exercise-induced changes in hormones could not explain the change in ligament structure or function. These data indicate that there is a significant gap in our understanding of muscle-connective tissue crosstalk. To address this gap, the current proposal seeks to: i) isolate and sequence exosomal RNA (long non-coding, miR, and mRNA) and ii) determine whether exosomes isolated from serum after exercise increase engineered ligament mechanics and collagen content.
Interventions
PROCEDUREResistance Exercise
Participants will perform a bout of lower body resistance exercise consisting of leg press, leg extension and leg curls.
Sponsors
University of California, Davis
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
TREATMENT
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
* Young healthy adults (18-35 y) * Experience with exercise (\>1y)
Exclusion criteria
* Pregnancy * Smoking * Receiving any medication that may interfere with the study outcomes
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Collagen content of ligament constructs | 15 min post-exercise | Ligament constructs will be treated for 7 days with experimental feed medium containing serum, or exosomes isolated from serum, obtained at baseline or post-exercise for determination of collagen content using a hydroxyproline assay. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Ultimate tensile stress and modulus of ligament constructs | 15 min post-exercise | ligament constructs will be treated for 7 days with experimental feed medium containing serum, or exosomes isolated from serum, obtained at baseline or post-exercise for determination of ultimate tensile stress and modulus. |
| Exosomal RNA | 15 min post-exercise | Isolation and sequencing of exosomal RNA from serum obtained at baseline or post-exercise. |
Countries
United States
Outcome results
None listed