Vitamin B 12 Deficiency
Conditions
Keywords
Vitamin B12, Vegetarian diet, Purple laver (Porphyra sp.), Nori
Brief summary
This is an intervention study aiming to understand the bioavailability of vitamin B12 from nori for vegetarians.
Detailed description
Thirty vegetarian participants will be randomly assigned to one of three intervention arms: (1) 5g of nori (4 sheets, containing 2.4μg vitamin B12), (2) 8g nori (7 sheets, containing 4μg vitamin B12), (3) control group (no nori). At baseline and end of 4 weeks, participants will be measured on serum vitamin B12, methylmalonic acid, holotranscobalamin, homocysteine and folate.
Interventions
OTHERDried purple laver (Nori)
Dried purple laver (Nori) that has been test to have bioactivity vitamin B12 will be given for intervention groups.
Sponsors
Fu Jen Catholic University
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
PREVENTION
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
20 Years to 60 Years
Healthy volunteers
Yes
Inclusion criteria
* Vegetarians (including vegan, ovo-vegetarian, lacto-vegetarian, lacto-ovo vegetarian) * Duration of the vegetarian diet: at least 1 year * Aged 20-60 years old * Have not taken vitamin B12 or folate supplement within 1 year * Willing to adhere to study intervention and be tested for vitamin B12 related biomarkers. * Familiar with LINE application on smartphone
Exclusion criteria
* Any disease or surgery that affects the vitamin B12 status (Pernicious anemia, Gastrointestinal disease and surgery, Pancreatic diseases, Chronic kidney disease) * Hyperthyroidism * Previous adverse reaction to nori * Have taken antacid or metformin in the past 1 week * Alcoholic and not willing to abstinence from alcoholic beverages
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Change in holotranscobalamin in 4 weeks | 4 weeks (Week 4 - Baseline) | Assessing the change in serum holotranscobalamin after consuming varying doses of nori |
| Change in methylmalonic acid in 4 weeks | 4 weeks (Week 4 - Baseline) | Assessing the change in serum methylmalonic acid after consuming varying doses of nori |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Change in serum vitamin B12 concentration in 4 weeks | 4 weeks (Week 4 - Baseline) | Assessing the change in serum vitamin B12 after consuming varying doses of nori |
| Change in homocysteine in 4 weeks | 4 weeks (Week 4 - Baseline) | Assessing the change in serum homocysteine after consuming varying doses of nori |
Countries
Taiwan
Outcome results
None listed