Nutrition Disorders in Old Age
Conditions
Brief summary
The investigators aim to assess 24-hour protein kinetics at two different levels of protein intake: 0.8 (RDA) and 1.6g/kg/day (2RDA) during a normal dietary (TRAD) and a time restricted feeding (TRF) pattern in older adults.
Detailed description
The investigators aim to assess 24-hour protein kinetics at two different levels of protein intake: 0.8 (RDA) and 1.6g/kg/day (2RDA) during a normal dietary (TRAD) and a time restricted feeding (TRF) pattern in older adults. Individuals in the TRF pattern will only consume food during a 6-hour eating period and fast for the remaining 18 hours. Individuals in the TRAD pattern will consume isocaloric and isonitrogenous (RDA or 2RDA) diet but consume an all-beef-breakfast. Participants will consume oral stable isotopes to measure muscle protein synthesis (MPS), whole-body muscle protein breakdown (MPB), and whole-body protein balance (WBPB). Appropriate muscle, blood samples, and urine will be collected throughout a 24-hour metabolic trial following a 2-day controlled diet.
Interventions
OTHERisocaloric, isonitrogenous meals
Participants will eat prepared study meals for 3 days that have either the RDA of protein per day or twice the RDA of protein per day.
Sponsors
University of Arkansas
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
OTHER
Masking
DOUBLE (Investigator, Outcomes Assessor)
Eligibility
Sex/Gender
ALL
Age
50 Years to 75 Years
Healthy volunteers
Yes
Inclusion criteria
1. Men and women ages 50-75 years. 2. BMI 20-30 kg/m2 inclusive. 3. Capable of providing informed consent. 4. COVID-19 negative and/or asymptomatic
Exclusion criteria
1. Subject who does not/will not eat animal protein sources. 2. Body mass index \<20 or \>30. 3. Hemoglobin \<10g/dL at screening. 4. Platelets \<150,000/uL at screening. 5. History of diabetes. 6. History of chemotherapy or radiation therapy for cancer in the 6 months prior to enrollment. 7. History of gastrointestinal bypass/reduction surgery. 8. History of a chronic inflammatory disease (e.g. what?) 9. Currently receiving androgen (e.g., testosterone) or anabolic (e.g., GH, IGF-I) therapy. 10. Currently using prescription blood thinning medications. 11. Currently using corticosteroid medications. 12. Unable or unwilling to suspend aspirin use for 7 days prior to Visit 3. 13. Unwilling to avoid using protein or amino-acid supplements during participation. 14. Subjects who are unwilling to fast overnight. 15. Participating in \>200 minutes/week of vigorous exercise and/or \>4 days/week of resistance training.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Whole-body protein balance | 24 hours | Volume of net protein produced per unit of time |
Countries
United States
Outcome results
None listed