Gene Polymorphisms, Skeletal Muscle Performance
Conditions
Keywords
Sport performance, gene polymorphism, N-acetylcysteine
Brief summary
Study aims to verify the hypothesis: * N-acetylcysteine (NAC) supplementation improves exercise capacity in people training endurance sports and the effectiveness of this supplementation depends on the polymorphism of genes related to folate and glutathione metabolism. * NAC supplementation affects the parameters of oxidative stress among women and men training endurance disciplines
Detailed description
* Glutathione depletion caused by a combination of intense physical exertion associated with the generation of significant amounts of reactive oxygen species (ROS), and possibly with having an unfavorable variant of the methylenetetrahydrofolate reductase (MTHFR), glutamate-cysteine ligase catalytic subunit (GCLC); glutamate-cysteine ligase modifier subunit (GCLM); glutathione S-transferase Pi 1 (GSTP1); glutathione synthetase (GSS) or cystathionine-β-synthase (CBS) genes may contribute to lower adaptation to exercise, but also to deterioration of health of people training endurance sports. * The main objective of the study is to verify the hypothesis that NAC supplementation has a beneficial effect on regeneration between intense exercise tests simulating a race characteristic for a given discipline. In addition, this study is aimed at checking whether genotype may modify the effectiveness of the supplementation measured as exercise and biochemical parameters . A double-blind study involving 100 women and men supplementing 1200 mg of N-acetylcysteine daily or 1200 mg of rice flour in three equal doses. Supplementation will last for 7 days. Blood sampling and body composition testing will be performed in the morning of the exercise test day. Discipline-specific exercise testing will be performed before and after the intervention.
Interventions
DIETARY_SUPPLEMENTN-acetylcysteine
100 women and men supplementing 1200 mg of N-acetylcysteine daily in three equal doses. Supplementation will last for 7 days.
DIETARY_SUPPLEMENTPlacebo
100 women and men supplementing 1200 mg of placebo daily in three equal doses. Supplementation will last for 7 days.
Sponsors
Poznan University of Life Sciences
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
DOUBLE (Subject, Investigator)
Masking description
Double (Participant/investigator)
Intervention model description
Eligible volunteers will be randomly assigned to one of the research groups. One of the groups will be taking the supplement for a period of 7 days, while the other group will be taking a placebo. Then a 3 week washout period will be performed. After that the groups will be exchanged and each participant will be assigned to a different group than it was in the first period of the study.
Eligibility
Sex/Gender
ALL
Age
18 Years to 45 Years
Healthy volunteers
Yes
Inclusion criteria
* Consent to participate in the study and acceptance of the established scheme for conducting the intervention * age range between 18 and 45 years old * regular training with a frequency of at least 3 times a week during the last year,
Exclusion criteria
* no consent to participate in the study and no acceptance of the established scheme of the conducted intervention * existing diseases of the blood system and neoplastic diseases * respiratory system diseases * obesity * any medical contraindications to exercise * Results obtained from exercise tests that do not meet the inclusion criteria * use of NAC during the last month * allergy to NAC * smoking * pregnancy and lactation
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Assesment of change in results of time trial test | Baseline,1 week | (min/test) change within groups and between groups |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Reduced glutathione (GSH) | Baseline,1 week | GSH (µM) concentrations change within the group and between the groups |
| Oxidized glutathione (GSSG) | Baseline,1 week | GSSG (µM) concentrations change within the group and between the groups |
| Homocysteine (Hcy) | Baseline,1 week | Hcy (µmol/l) concentrations change within the group and between the groups |
| Total cholesterol (TChol) | Baseline,1 week | TChol (mg/dl) concentrations change within the group and between the groups |
| Blood HDL-cholesterol (HDL-C) | Baseline,1 week | HDL-C (mg/dl) concentrations change within the group and between the groups |
| Blood LDL-cholesterol (LDL-C) | 1 week | LDL-C (mg/dl) concentrations change within the group and between the groups |
| Blood triacylglycerol (TAG) | Baseline,1 week | TAG (mg/dl) concentrations change within the group and between the groups |
| Dietary intake | Baseline,1 week | macro and micronutrient intake (g,mg,ug) |
| aspartate aminotransferase (ASPAT) | 1 week | ASPAT \[U/l\] Changes within groups and between groups |
| Alanine transaminase (ALAT) | Baseline,1 week | ALAT \[U/l\] change within groups and between groups |
| Oxygen volume (VO2) | Baseline,1 week | VO2 (ml/kg bw./min) change within groups and between groups |
| Carbon dioxide volume (VCO2) | Baseline,1 week | VCO2 (ml/kgm.c./min) change within groups and between groups |
| Testosterone (T) | Baseline,1 week | T (ng/dL) concentrations change within the group and between the groups |
| Cortisol (C) | Baseline,1 week | C (ng/dL) concentrations change within the group and between the groups |
| Body mass(BM) | Baseline, 1 week | Change in BM (kg) within groups and between groups |
| Fat Free Mass (FFM) | Baseline, 1 week | FFM change within (kg) groups and between groups |
| Fat Mass% (FM%) | Baseline, 1 week | FM% change within groups and between groups |
| Thiobarbituric Acid Reactive Species (TBARS) | 1 week | TBARS concentrations change within the group and between the groups |
Countries
Poland
Contacts
PRINCIPAL_INVESTIGATORMarcin Sadowski, MSc
Poznań University of Life Science
Outcome results
None listed