Atopic Dermatitis
Conditions
Keywords
tape strips, endotype
Brief summary
This study will be use the tape strip technique to evaluate the skin biomarkers of atopic dermatitis among Thai patients to differentiate clinical phenotype.
Detailed description
The patients will be enrolled in this study if they have been diagnosed with atopic dermatitis. All participants (AD patients and controls) will be evaluated their skin biomarkers by using tape stripping. The tape strips will be applied to the antecubital fossa to collect the epithelial samples. Then RNA was extracted from the tape strips for mRNA profiling to identify the immune and epidermal barrier genes.
Interventions
GENETICTape strips
Tape strip is a minimally invasive method that captures the stratum corneum. It is use to identify skin biomarkers in atopic dermatitis.
Sponsors
Mahidol University
Icahn School of Medicine at Mount Sinai
Samitivej Hospital group
Queen Sirikit National Institute of Child Health
Study design
Allocation
NON_RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
1 Years to 60 Years
Healthy volunteers
Yes
Inclusion criteria
* Children (age 1-18 years old) with mild atopic dermatitis * Children (age 1-18 years old) with moderate to severe atopic dermatitis * Children (age 1-18 years old) with moderate to severe atopic dermatitis and food allergy * Adult (age 18-60 years old) with atopic dermatitis * Healthy individuals (1-60 years old) * Patients with asthma (1-60 years old)
Exclusion criteria
* Active skin infections * Used systemic immunosuppressants within 4 weeks * Used topical steroids or immunomodulators within 1 week * Used moisturizers within 12 hours before evaluation
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Cellular AD biomarkers | 1 month | Evaluated by RNA sequencing: RNA was extracted for real-time polymerase chain reaction (RT-PCR) with the miRNAeasy Mini Kit (Qiagen, Hilden, Germany). Reverse transcription to complementary DNA (cDNA) from RNA was carried out using the High Capacity cDNA reverse transcription (Thermo fisher). TaqMan Low Density Array (TLDA) cards (Thermo fisher) were used for quantitative reverse transcription polymerase chain reaction (qRT-PCR). 500pg total RNA was used for PreAMP pool. Eukaryotic 18S recombinant RNA (rRNA) was used as an endogenous control. Expression values were normalized to Rplp0 |
| Immune biomarkers | 1 month | Evaluated by RNA sequencing: RNA was extracted for real-time polymerase chain reaction (RT-PCR) with the miRNAeasy Mini Kit (Qiagen, Hilden, Germany). Reverse transcription to complementary DNA (cDNA) from RNA was carried out using the High Capacity cDNA reverse transcription (Thermo fisher). TaqMan Low Density Array (TLDA) cards (Thermo fisher) were used for quantitative reverse transcription polymerase chain reaction (qRT-PCR). 500pg total RNA was used for PreAMP pool. Eukaryotic 18S recombinant RNA (rRNA) was used as an endogenous control. Expression values were normalized to Rplp0 |
| Barrier biomarkers | 1 month | chain reaction (RT-PCR) with the miRNAeasy Mini Kit (Qiagen, Hilden, Germany). Reverse transcription to complementary DNA (cDNA) from RNA was carried out using the High Capacity cDNA reverse transcription (Thermo fisher). TaqMan Low Density Array (TLDA) cards (Thermo fisher) were used for quantitative reverse transcription polymerase chain reaction (qRT-PCR). 500pg total RNA was used for PreAMP pool. Eukaryotic 18S recombinant RNA (rRNA) was used as an endogenous control. Expression values were normalized to Rplp0 |
Countries
Thailand
Contacts
Primary ContactPanipak Temboonnark, MD
Backup ContactTassalapa Daengsuwan, MD
Outcome results
None listed