Healthy
Conditions
Keywords
Zinc, gut microbiota, healthy adult
Brief summary
In this A Single-center, Prospective, Self-controlled trial, subjects should avoid a high-Zinc diet for 2 weeks and then take Zinc supplements for 2 weeks.
Interventions
DIETARY_SUPPLEMENTZinc Gluconate
Each participant should receive a regular zinc diet for weeks 1-2 and take zinc supplements for weeks 3-4.
Sponsors
The Seventh Affiliated Hospital, Southern Medical University
Study design
Allocation
NA
Intervention model
SINGLE_GROUP
Primary purpose
BASIC_SCIENCE
Masking
NONE
Eligibility
Sex/Gender
ALL
Age
20 Years to 30 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy adults aged 20-30 years from the general community were recruited to participate in the study voluntarily without underlying diseases.
Exclusion criteria
Patients who have taken antibiotics orally in the past 3 months or have acute or chronic inflammation; Those who have taken hormones within 3 months; Have taken any drugs that may interfere with glucose and lipid metabolism (such as insulin, glyburide, indomethacin, phentolamine, fuuside, phenytoin sodium, cortisone, etc.) and have had probiotic intervention within 1 month; smokers and drinkers; Uncontrollable mental disorders (including hospitalization history of mental illness); (6) Currently attending a weight loss or weight management course; prescribed diet for specific or other reasons (e.g. celiac disease); pregnant or lactating women; Patients with cardiac and renal insufficiency; Patients with other underlying diseases; Long-term constipation.
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| 16S rDNA sequencing | 14days，28 days | 16S rDNA sequencing technology was used to identify the structural and functional changes of intestinal microbiota before and after Zinc supplementation. |
| metabolomic analysis of serum and feces | 14days，28 days | To determine the alteration of gut microbiota after Zn supplementary. |
| The blood levels of Zn2+ in each study phase | 14days，28 days | The blood levels of Zn2+ would be detected on day 14 and day 28. |
| Number of participantswith abnormal laboratory tests results | 14days，28 days | If there are abnormal situations during the experiment that may pose significant risks to the health or safety of the participants, the research team may need to terminate the experiment to ensure participant safety. Only |
| The fecal Zn2+ concentration in each study phase | 14days，28 days | The fecal Zn2+ concentration would be detected on day 14 and day 28. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| total protein concentration after 14days oral Zn. | 14days，28 days | change from 14days in total protein and analyzed by the AU5800 fully automatic biochemical analyzer. |
| aspartate transaminase concentration after 14days oral Zn. | 14days，28 days | change from 14days in aspartate transaminase and analyzed by the AU5800 fully automatic biochemical analyzer. |
| alanine transaminase concentration after 14days oral Zn. | 14days，28 days | change from 14days in alanine transaminase and analyzed by the AU5800 fully automatic biochemical analyzer. |
| albumin concentration after 14days oral Zn. | 14days，28 days | change from 14days in albumin and analyzed by the AU5800 fully automatic biochemical analyzer. |
| total cholesterol concentration after 14days oral Zn. | 14days，28 days | change from 14days in total cholesterol and analyzed by the AU5800 fully automatic biochemical analyzer. |
| Urea concentration after 14days oral Zn. | 14days，28 days | change from 14 days in urea and analyzed by the AU5800 fully automatic biochemical analyzer. |
| HDL-C concentration after 14days oral Zn. | 14days，28 days | change from 14 days in HDL-C(high density lipoprotein cholesterol) and analyzed by the AU5800 fully automatic |
| LDL-C concentration after 14days oral Zn. | 14days，28 days | change from 14 days in LDC-C(low density lipoprotein cholesterol) and analyzed by the AU5800 fully automatic |
| Total bilirubin concentration after 14days oral Zn. | 14days，28 days | change from 14days in Total bilirubin and analyzed by the AU5800 fully automatic biochemical analyzer. |
| Plateltes analysis after 14days oral Zn. | 14days，28 days | change from 14 days in Plateltes and determined by a Sysmex-xn-2000 automatic blood cell analyser. |
| Neutrophil cells analysis after 14days oral Zn. | 14days，28 days | change from 14days in Neutrophil cells and determined by a Sysmex-xn-2000 automatic blood cell analyser. |
| Eosinophil cells analysis after 14days oral Zn. | 14days，28 days | change from 14days in Eosinophil cells and determined by a Sysmex-xn-2000 automatic blood cell analyser. |
| Basophil cells analysis after 14days oral Zn. | 14days，28 days | change from 14 days in Basophil cells and determined by a Sysmex-xn-2000 automatic blood cell analyser. |
| Lymphocyte cells analysis after 14days oral Zn. | 14days，28 days | change from 14 days in Lymphocyte cells and determined by a Sysmex-xn-2000 automatic blood cell analyser. |
| Monocyte cells analysis after 14days oral Zn. | 14days，28 days | change from 14 days in Monocyte cells and determined by a Sysmex-xn-2000 automatic blood cell analyser. |
| White blood cells analysis after 14days oral Zn. | 14days，28 days | change from 14 days in white blood cells and determined by a Sysmex-xn-2000 automatic blood cell analyser. |
| Red blood cells analysis after 14days oral Zn. | 14days，28 days | change from 14days in Red blood cells and determined by a Sysmex-xn-2000 automatic blood cell analyser. |
| Direct bilirubin concentration after 14days oral Zn. | 14days，28 days | change from 14days in Direct bilirubin and analyzed by the AU5800 fully automatic biochemical analyzer. |
| Indirect bilirubin concentration after 14days oral Zn. | 14days，28 days | change from 14days in indirect bilirubin and analyzed by the AU5800 fully automatic biochemical analyzer. |
| uric acid concentration after 14days oral Zn. | 14days，28 days | change from 14days in uric acid and analyzed by the AU5800 fully automatic biochemical analyzer. |
| total triglyceride concentration after 14days oral Zn. | 14days，28 days | change from 14days in total triglyceride and analyzed by the AU5800 fully automatic biochemical analyzer. |
Countries
China
Outcome results
None listed