Inflammation
Conditions
Keywords
Exercise, Cytokines, Leukocytes, Interleukin-10, Interleukin-6
Brief summary
The immune system helps prevent illness, fights off infections, and repairs damaged tissues following an injury. However, when immune cells remain active for prolonged periods of time - a state known as chronic inflammation - they can contribute to the development and progression of chronic diseases like heart disease and diabetes. Exercise can reduce the risk of developing many of these diseases and at least part of the health benefits of exercise are due to the ability of exercise to reduce chronic inflammation. The inflammation-lowering effects of exercise are typically captured by measuring hormone-like molecules released from immune cells called cytokines in the blood. In addition to changes in circulating cytokine levels, exercise may also alter how immune cells respond to these cytokines. How exercise intensity (i.e., how hard you are working during exercise) and pattern (i.e., exercising as a long continuous bout or in short intervals) impact the ability of immune cells to respond to cytokines is not well understood. A better understanding of how exercise intensity and pattern of exercise for reducing chronic inflammation may help determine the best types of exercises for improving health and preventing chronic diseases.
Interventions
Participants will perform an acute bout of interval cycling at at 10% of the difference between lactate threshold and VO2peak until an energy expenditure of 350 kcal is achieved. Blood samples will be obtained immediately before and immediately, 30, and 90 minutes after exercise.
OTHERResting (no exercise) control
Participants will remain in a rested state (i.e., no exercise) for the entire session. Blood samples will be obtained at the same time-points as the exercise sessions.
Participants will perform an acute bout of continuous cycling at 70% of the power output at lactate threshold until an energy expenditure of 350 kcal is achieved. Blood samples will be obtained immediately before and immediately, 30, and 90 minutes after exercise.
Participants will perform an acute bout of continuous cycling at 10% of the difference between lactate threshold and VO2peak until an energy expenditure of 350 kcal is achieved. Blood samples will be obtained immediately before and immediately, 30, and 90 minutes after exercise.
Sponsors
University of British Columbia
Study design
Allocation
RANDOMIZED
Intervention model
CROSSOVER
Primary purpose
BASIC_SCIENCE
Masking
NONE
Intervention model description
Repeated measures randomized counterbalanced crossover design
Eligibility
Sex/Gender
ALL
Age
18 Years to 35 Years
Healthy volunteers
Yes
Inclusion criteria
* 18-35 years of age * Body mass index between 18.5-30 kg/m\^2 * Free of cardiometabolic and autoimmune/inflammatory disease
Exclusion criteria
* Competitive endurance athlete * Cigarette smoker * Currently taking immunomodulatory/anti-inflammatory medications * Currently pregnant
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| IL-10 mediated STAT3 phosphorylation | Change from pre-exercise to immediately and 90-min post-exercise | Ex vivo leukocyte STAT3 phosphorylation in response to IL-10 treatment |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| IL-6 mediated STAT3 phosphorylation | Change from pre-exercise to immediately and 90-min post-exercise | Ex vivo leukocyte STAT3 phosphorylation in response to IL-6 treatment |
| IL-6 mediated TNF-alpha inhibition | Change from pre-exercise to immediately and 90-min post-exercise | Ex vivo inhibition of TNF-alpha production in response to IL-6 treatment |
| Plasma IL-10 | Change from pre-exercise to immediately, 30-, and 90-min post-exercise | Concentration of IL-10 in plasma samples |
| IL-10 mediated TNF-alpha inhibition | Change from pre-exercise to immediately and 90-min post-exercise | Ex vivo inhibition of TNF-alpha production in response to IL-10 treatment |
| Plasma TNF-alpha | Change from pre-exercise to immediately, 30-, and 90-min post-exercise | Concentration of TNF-alpha in plasma samples |
| Hematology panel | Change from pre-exercise to immediately, 30-, and 90-min post-exercise | Complete blood count |
| Extracellular vesicles | Change from pre-exercise to immediately, 30-, and 90-min post-exercise | Concentration of extracellular vesicles in plasma |
| Plasma IL-6 | Change from pre-exercise to immediately, 30-, and 90-min post-exercise | Concentration of IL-6 in plasma samples |
Countries
Canada
Outcome results
None listed