Aging, Exercise
Conditions
Brief summary
The incidence of age-related diseases, such as cancer and cardiovascular diseases, rapidly rises. These days, age-related diseases cause the majority of all healthcare expenses. During ageing senescent cells secrete a range of harmful inflammatory signals, which leads to chronic inflammation (inflamm-aging) and accelerates aging throughout the rest of the body. Interestingly, people who regularly exercise show less signs of inflamm-aging compared to people with a sedentary lifestyle. Molecules secreted by skeletal muscles, called myokines, may play an important role. However, the underlying molecular mechanisms remain unknown. This pilot study aims to investigate if an acute bout of resistance training is an appropriate tool to induce an increase in the release of muscle derived myokines in aged persons.
Interventions
PROCEDUREPercutaneous muscle biopsy
Muscle biopsy of the vastus lateralis (m. quadriceps)
OTHERExercise training
Resistance training of the lower limbs
BEHAVIORALQuestionnaire
Physical activity questionnaire
PROCEDUREBlood sample
Venous blood sample
Sponsors
Hasselt University
Study design
Allocation
RANDOMIZED
Intervention model
PARALLEL
Primary purpose
BASIC_SCIENCE
Masking
SINGLE (Outcomes Assessor)
Intervention model description
Aged individuals who perform exercise training are compared to aged individuals who remain sedentary.
Eligibility
Sex/Gender
ALL
Age
65 Years to 85 Years
Healthy volunteers
Yes
Inclusion criteria
* Healthy persons
Exclusion criteria
* Smoking * Infection \< 2 weeks ago * Received a vaccine \< 2 weeks ago * Allergy to Lidocain * Immune deficiency * Presence of a tumour or in treatment * Usage of anti-coagulant medication * Presence of following conditions: * Haemophilia * Auto-immune disease * Cardiovascular disease * Musculoskeletal diseases which refrains the patient from exercising
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| Myokine gene expression | Day 2 | Quantitative PCR (polymerase chain reaction) of markers in muscle tissue |
| Myokine production | Day 2 | Immunohistochemistry staining (fluorescent) of multiple myokines (for example: IL-6, IL-15, IL-4, irisin..) and aging markers (p16,p21...) on muscle tissue |
| Immune parameters | Day 2 | Flow cytometry panels of immune parameters in muscle tissue |
| Myokine protein levels | Day 2 | Western blotting of multiple myokines in muscle tissue |
| Cytokine concentration | Day 2 | Measurement of multiple cytokines in blood (for example: IL-6, IL-15, IL-4, irisin...) with ELISA's or multiplex assays |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| Resting Heart Rate | Day 1 | Measured using an electronic sphygmomanometer |
| International Physical Activity Questionnaire (Short form) | Day 1 | Measures the subjective level of activity of the last seven days. 7 questions regarding amount of time spent on certain activities. |
| Blood pressure | Day 1 | The force of circulating blood on the walls of the arteries. Measured using an electronic sphygmomanometer |
Countries
Belgium
Contacts
Primary ContactBert Op'T Eijnde, Prof. dr.
Backup ContactChiel Hex, MD.
Outcome results
None listed