SARS-CoV-2 Infection
Conditions
Keywords
COVID-19; SARS-CoV-2
Brief summary
The aim of the study is to characterize and to quantify SARS-CoV-2 strains in stools, in comparison with the viral strains in respiratory samples, from patients hospitalized for COVID-19. The methods will be standard ddPCR quantifying SARS-CoV-2 RNA and next generation sequencing-whole genome sequencing. The expected results will allow us to better understand SARS-CoV-2 dynamics and compartmentalization, both in the respiratory tract and in digestive-related tissues, according to the evolution of SARS-CoV-2 variants.
Interventions
DIAGNOSTIC_TESTBiological assays
PCR and NGS-WGS on stools and respiratory samples
Sponsors
Central Hospital, Nancy, France
Study design
Observational model
CASE_ONLY
Time perspective
PROSPECTIVE
Eligibility
Sex/Gender
ALL
Age
18 Years to 90 Years
Healthy volunteers
No
Inclusion criteria
* Age: men or women of 18 years-old or more * Clinical signs: hospitalised patient for SARS-CoV-2 infection, nasopharyngeal PCR-confirmed (or any other sample) (in: Infectious diseases department, Intensive care unit), whatever the severity of the clinical form. * The subject gave his/her informed and writen consent, after complete explanations on the research schedule. * Compulsory affiliation to a social security scheme. * Previous medical examination, when a patient is admitted to the hospital, according to good clinical practices.
Exclusion criteria
* Subject corresponding to articles L. 1121-5, L. 1121-7 and L1121-8 of the French Public health code: * pregnant woman, parturient or breastfeeding woman * Minor person * Person of full age under guardianship, curatorship, safeguard of justice * Woman who wants to be pregnant during the study period * Person in psychiatric care - articles L. 3212-1 and L. 3213-1 who do not fall under the article L. 1121-8 (without consent hospitalisation)
Design outcomes
Primary
| Measure | Time frame | Description |
|---|---|---|
| SARS-CoV-2 dynamics in stools. | 30 monthes | Evolutive SARS-CoV-2 dynamics in stools compared to respiratory tract: molecular quantification (ddPCR) of SARS-CoV-2 variants genome in stools, compared to the viral quantification in respiratory samples. |
Secondary
| Measure | Time frame | Description |
|---|---|---|
| SARS-CoV-2 in stools compared to the virus in wastewaters. | 30 monthes | SARS-CoV-2 in stools compared to wastewaters: molecular quantification (ddPCR) of SARS-CoV-2 variants genome in stools, compared to the viral quantification in wastewater samples. |
| Modelization of SARS-CoV-2 load in stools and COVID-19 global frequency. | 30 monthes | SARS-CoV-2 load in stools and COVID-19 global frequency: correlation between SARS-CoV-2 load in stools by ddPCR and COVID-19 incidence in the neighboring global inhabitants population. |
Countries
France
Contacts
Primary ContactEl Mehdi MS Siaghy
Outcome results
None listed